Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lind, T.; Lindahl, U.; Lidholt, K.
    Biosynthesis of heparin/heparan sulfate. Identification of a 70-kDa protein catalyzing both the D-glucuronosyl- and the N-acetyl-D-glucosaminyltransferase reactions (1993), J. Biol. Chem., 268, 20705-20708.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.225 Golgi apparatus
-
 Bos taurus 5794
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.224 UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan Bos taurus elongation of growing chains of heparin and heparan sulfate, tumor suppressor UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?
2.4.1.225 UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-proteoglycan Bos taurus
-
 UDP + beta-glucuronosyl-(1-4)-N-acetyl-alpha-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-proteoglycan
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.224 Bos taurus
-
-
-
2.4.1.225 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.225
-
 Bos taurus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.225 additional information
-
 6700000 dpm 14C/mg Bos taurus
2.4.1.225 additional information
-
 12000000 dpm 3H/mg Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.224 additional information EXT1 and EXT2 proteins also acts as beta(1,4)-glucuronyltransferase (EC 2.4.1.225) Bos taurus ?
-
 ?
2.4.1.224 UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 Bos taurus UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?
2.4.1.224 UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan elongation of growing chains of heparin and heparan sulfate, tumor suppressor Bos taurus UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?
2.4.1.224 UDP-N-acetyl-D-glucosamine + [D-glucuronosyl-beta-(1->4)-N-acetyl-D-glucoseamin-alpha-(1->4)]n-D-glucuronosyl-2,5-anhydro-D-mannose octasaccharide but not nonasaccharide serves as acceptor Bos taurus UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-[D-glucuronosyl-beta-(1->4)-N-acetyl-D-glucoseaminyl-alpha-(1->4)]n-D-glucuronosyl-2,5-anhydro-D-mannose
-
 ?
2.4.1.225 UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-proteoglycan
-
 Bos taurus UDP + beta-glucuronosyl-(1-4)-N-acetyl-alpha-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-proteoglycan
-
 ?