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Literature summary extracted from

  • Minami, Y.; Nishimura, O.; Hara-Nishimura, I.; Nishimura, M.; Matsubara, H.
    Tissue and intracellular localization of indican and the purification and characterization of indican synthase from indigo plants (2000), Plant Cell Physiol., 41, 218-225.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.220 0.13
-
 UDP-glucose
-
 Persicaria tinctoria

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.220 vacuole
-
 Persicaria tinctoria 5773
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.220 48000
-
 gel-filtration Persicaria tinctoria
2.4.1.220 53000
-
 SDS-PAGE Persicaria tinctoria

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.220 Persicaria tinctoria
-
 indigo plant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.220
-
 Persicaria tinctoria

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.220 leaf
-
 Persicaria tinctoria
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.220 ADP-glucose + indoxyl
-
 Persicaria tinctoria ADP + indican
-
 ?
2.4.1.220 GDP-glucose + indoxyl
-
 Persicaria tinctoria GDP + indican
-
 ?
2.4.1.220 UDP-glucose + indoxyl best substrate Persicaria tinctoria UDP + 1H-indole-3-yl-beta-D-glucopyranoside i.e. indican ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.220 30 50 30 and 50 half-maximal-activity Persicaria tinctoria

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.220 10
-
-
 Persicaria tinctoria