EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.219 | D-fructose | - |
Rauvolfia serpentina | |
2.4.1.219 | D-glucose | - |
Rauvolfia serpentina | |
3.2.1.125 | D-fructose | 0.95 M: 30% inhibition | Rauvolfia serpentina | |
3.2.1.125 | D-glucose | 0.8 M: complete inhibition | Rauvolfia serpentina | |
3.2.1.125 | additional information | D-glucono-1,5-lactone up to 1 M, phenylmethylsulfonyl fluoride up to 80 mM, EDTA up to 80 mM, iodoacetamide up to 80 mM, strictosidine or amygdalin up to 50 mM, mannitol up to 1 M are no inhibitors | Rauvolfia serpentina |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.125 | 1.4 | - |
Raucaffricine | - |
Rauvolfia serpentina | |
3.2.1.125 | 1.4 | - |
1,2-Dihydro-1-methylraucaffricine | - |
Rauvolfia serpentina | |
3.2.1.125 | 1.5 | - |
1,2-Dihydroraucaffricine | - |
Rauvolfia serpentina | |
3.2.1.125 | 1.7 | - |
17-O-Deacetyl-1,2-dihydroraucaffricine | - |
Rauvolfia serpentina | |
3.2.1.125 | 2 | - |
1,2,19,20-Tetrahydroraucaffricine | - |
Rauvolfia serpentina |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.219 | microsome | - |
Rauvolfia serpentina | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.219 | 66600 | - |
gel filtration | Rauvolfia serpentina |
3.2.1.125 | 66600 | - |
gel filtration | Rauvolfia serpentina |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.219 | UDP-glucose + vomilenine | Rauvolfia serpentina | - |
UDP + raucaffricine | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.219 | Rauvolfia mannii | - |
- |
- |
2.4.1.219 | Rauvolfia serpentina | - |
- |
- |
2.4.1.219 | Rauvolfia verticillata | - |
- |
- |
2.4.1.219 | Rauwolfia caffra | - |
- |
- |
3.2.1.125 | Rauvolfia caffra | - |
- |
- |
3.2.1.125 | Rauvolfia mannii | - |
- |
- |
3.2.1.125 | Rauvolfia serpentina | - |
- |
- |
3.2.1.125 | Rauvolfia verticillata | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.125 | partial | Rauvolfia serpentina |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.219 | root | - |
Rauvolfia serpentina | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.219 | additional information | - |
538 pkat/mg | Rauvolfia serpentina |
2.4.1.219 | additional information | - |
52 pkat/mg | Rauvolfia mannii |
2.4.1.219 | additional information | - |
253 pkat/mg | Rauwolfia caffra |
2.4.1.219 | additional information | - |
136 pkat/mg | Rauvolfia verticillata |
3.2.1.125 | 22.5 | - |
reaction with raucaffricine | Rauvolfia serpentina |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.1.219 | 4°C, 6 month, 30% loss in activity | Rauvolfia serpentina |
3.2.1.125 | 4°C, crude enzyme, 30% loss of activity within 6 months | Rauvolfia serpentina |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.219 | UDP-glucose + vomilenine | - |
Rauvolfia serpentina | UDP + raucaffricine | - |
r | |
3.2.1.125 | 1,2,19,20-tetrahydroraucaffricine + H2O | 87% of activity compared to raucaffricine | Rauvolfia serpentina | 17-O-acetylnorajmaline + D-glucose | - |
? | |
3.2.1.125 | 1,2-dihydro-1-methylraucaffricine + H2O | 36% of activity compared to raucaffricine | Rauvolfia serpentina | acetic acid 3-ethylidene-4-hydroxy-13-methyl-1,3,4,7,8,13,13a,13b-octahydro-2H,6H-2,7-cyclo-6,8a-methano-pyrido[1',2':1,2]azepino[3,4-b]indol-8-yl ester + D-glucose | - |
? | |
3.2.1.125 | 1,2-dihydroraucaffricine + H2O | 98% of activity compared to raucaffricine | Rauvolfia serpentina | 2beta(R)-1,2-dihydrovomilenine + D-glucose | - |
? | |
3.2.1.125 | 17-O-deacetyl-1,2-dihydroraucaffricine + H2O | 95% of activity compared to raucaffricine | Rauvolfia serpentina | 3-ethylidene-1,3,4,7,8,13,13a,13b-octahydro-2H,6H-2,7-cyclo-6,8a-methano-pyrido[1',2':1,2]azepino[3,4-b]indole-4,8-diol + D-glucose | - |
? | |
3.2.1.125 | raucaffricine + H2O | - |
Rauvolfia serpentina | vomilenine + D-glucose | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.219 | 38 | - |
- |
Rauvolfia serpentina |
3.2.1.125 | 38 | - |
- |
Rauvolfia serpentina |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.125 | 20 | 55 | 20°C: about 55% of maximal activity, 55°C: about 40% of maximal activity | Rauvolfia serpentina |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.219 | 55 | - |
30 min, 50% of activity left | Rauvolfia serpentina |
3.2.1.125 | 55 | - |
30 min, 50% loss of activity | Rauvolfia serpentina |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.219 | 5.1 | - |
- |
Rauvolfia serpentina |
2.4.1.219 | 6.3 | - |
- |
Rauvolfia serpentina |
3.2.1.125 | 5.1 | - |
- |
Rauvolfia serpentina |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.219 | 4 | 7 | - |
Rauvolfia serpentina |
3.2.1.125 | 4.2 | 6 | half-maximal activity at pH 4.2 and pH 6.0 | Rauvolfia serpentina |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.125 | Rauvolfia serpentina | isoelectric focusing | - |
5.8 |