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Literature summary extracted from

  • Vikstrom, S.; Li, L.; Karlsson, O.P.; Wieslander, A.
    Key role of the diglucosyldiacylglycerol synthase for the nonbilayer-bilayer lipid balance of Acholeplasma laidlawii membranes (1999), Biochemistry, 38, 5511-5520.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.208 1,2-dioleoylphosphatidylethanolamine maximal activity at 4.5% Acholeplasma laidlawii
2.4.1.208 1,2-dioleoylphosphatidylglycerol
-
 Acholeplasma laidlawii
2.4.1.208 1,2-dioleoylphosphatidylserine 33% of activation with 1,2-dioleoylphosphatidylglycerol Acholeplasma laidlawii
2.4.1.208 1,3-dioleoylglycerol 11.5 mol%, 5fold activation Acholeplasma laidlawii
2.4.1.208 diphosphatidylglycerol from bovine, 50% of activation with 1,2-dioleoylphosphatidylglycerol Acholeplasma laidlawii
2.4.1.208 phosphate 40 mM, approx. 8fold activation Acholeplasma laidlawii
2.4.1.208 phosphatidylglycerol enzyme needs a polar activator lipid, preferably phosphatidylglycerol at concentrations of 25-30 mol% in zwitterionic mixed CHAPS bilayer-like aggregates Acholeplasma laidlawii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.208 1,2-diacyl-3-O-(beta-D-galactopyranosyl)-sn-glycerol
-
 Acholeplasma laidlawii
2.4.1.208 1,2-diacyl-3-O-[6-O-acyl(alpha-D-glucopyranosyl)]-sn-glycerol
-
 Acholeplasma laidlawii
2.4.1.208 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-1,2-O-(6-O-acyl-alpha-D-glucopyranosyl)]-sn-glycerol
-
 Acholeplasma laidlawii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.208 0.14
-
 UDP-glucose
-
 Acholeplasma laidlawii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.208 Acholeplasma laidlawii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.208 solubilization, SP-Sepharose, hydroxyapatite, Green A agarose Acholeplasma laidlawii

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.4.1.208 enzyme can be reconstituted in liposome bilayers by the dialyse techique Acholeplasma laidlawii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.208 8.05
-
-
 Acholeplasma laidlawii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.208 UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol no activity if the sugar is in beta-position Acholeplasma laidlawii UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
 ?
2.4.1.208 UDP-glucose + 1,2-diheptadecanoyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol best substrate Acholeplasma laidlawii UDP + 1,2-diheptadecanoyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol 73% of activity with monoglucosyldioleoylglycerol ?
2.4.1.208 UDP-glucose + 1,2-dioleoyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
-
 Acholeplasma laidlawii UDP + 1,2-dioleoyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
 ?