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Literature summary extracted from

  • Yamamoto, T.; Shiraki, K.; Fujiwara, S.; Takagi, M.; Fukui, K.; Imanaka, T.
    In vitro heat effect on functional and conformational changes of cyclodextrin glucanotransferase from hyperthermophilic archaea (1999), Biochem. Biophys. Res. Commun., 265, 57-61.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.19
-
 Thermococcus sp.
2.4.1.19 cgtA gene cloned and expressed in Escherichia coli JM109 Thermococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Geobacillus stearothermophilus
-
-
-
2.4.1.19 Niallia circulans
-
-
-
2.4.1.19 Niallia circulans
-
 no. 8
-
2.4.1.19 Niallia circulans 251
-
-
-
2.4.1.19 Niallia circulans 8
-
 no. 8
-
2.4.1.19 Thermoanaerobacterium thermosulfurigenes
-
 EM1
-
2.4.1.19 Thermoanaerobacterium thermosulfurigenes EM1
-
 EM1
-
2.4.1.19 Thermococcus sp.
-
 B1001
-
2.4.1.19 Thermococcus sp. Q9UWN2 B1001, DNA sequence of CGTase gene, EMBL/GenBank/DDBJ database accession No.
-
2.4.1.19 Thermococcus sp. B1001
-
 B1001
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19 natural and recombinant enzyme Thermococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 soluble starch + glycosyl acceptor
-
 Thermococcus sp. alpha-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Thermococcus sp. B1001 alpha-cyclodextrin
-
 r
2.4.1.19 starch
-
 Thermococcus sp. beta-cyclodextrin
-
 ?
2.4.1.19 starch
-
 Thermococcus sp. B1001 beta-cyclodextrin
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.19 40 107
-
 Thermococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.19 80 110 above 80°C activity increases by heat treatment, enzyme is irreversibly unfolded above 110°C Thermococcus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.19 1
-
 starch unheated CGTase Thermococcus sp.
2.4.1.19 1.33
-
 starch heated CGTase Thermococcus sp.