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Literature summary extracted from

  • Jeang, C.L.; Wung, C.H.; Chang, B.Y.; Yeh, S.S.; Lour, D.W.
    Characterization of the Bacillus macerans cyclodextrin glucanotransferase overexpressed in Escherichia coli (1999), Proc. Natl. Sci. Counc. Repub. China B, 23, 62-68.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.19 cloned and overexpressed in Escherichia coli Paenibacillus macerans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.19 0.58
-
 starch wild-type Paenibacillus macerans
2.4.1.19 1.05
-
 starch mutant E344D Paenibacillus macerans
2.4.1.19 2.1
-
 starch authentic CGTase Paenibacillus macerans

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Paenibacillus macerans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19 wild-type and recombinant enzymes Paenibacillus macerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 alpha-1,4-glucan + glycosyl acceptor
-
 Paenibacillus macerans cyclodextrins
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Paenibacillus macerans cyclodextrins
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.19 0.442
-
 starch wild-type Paenibacillus macerans
2.4.1.19 1.51
-
 starch mutant E344D Paenibacillus macerans
2.4.1.19 1.66
-
 starch authentic CGTase Paenibacillus macerans