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Literature summary extracted from

  • Tomita, K.; Kaneda, M.; Kawamura, K.; Nakanishi, K.
    Purification and properties of a cyclodextrin glucanotransferase from Bacillus autolyticus 11149 and selective formation of beta-cyclodextrin (1993), J. Ferment. Bioeng., 75, 89-92.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.19 (CH3COO)2Pb relative activity 103% Bacillus autolyticus
2.4.1.19 2-mercaptoethanol relative activity 101% Bacillus autolyticus
2.4.1.19 dithiothreitol relative activity 101% Bacillus autolyticus
2.4.1.19 ethanol
-
 Geobacillus stearothermophilus
2.4.1.19 ethanol
-
 Bacillus sp. (in: Bacteria)
2.4.1.19 ethanol
-
 Niallia circulans
2.4.1.19 ethanol
-
 Paenibacillus macerans
2.4.1.19 ethanol enhances specificity and yield of beta-cyclodextrin production Bacillus autolyticus
2.4.1.19 Triton X-100 enhances specificity and yield of beta-cyclodextrin production Bacillus autolyticus

Application

EC Number Application Comment Organism
2.4.1.19 synthesis
-
 Geobacillus stearothermophilus
2.4.1.19 synthesis
-
 Bacillus sp. (in: Bacteria)
2.4.1.19 synthesis
-
 Niallia circulans
2.4.1.19 synthesis
-
 Paenibacillus macerans
2.4.1.19 synthesis used in industrial applications Bacillus autolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.19 HgCl2
-
 Bacillus autolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.19 68000
-
-
 Geobacillus stearothermophilus
2.4.1.19 68000
-
-
 Bacillus sp. (in: Bacteria)
2.4.1.19 68000
-
-
 Paenibacillus macerans
2.4.1.19 68000
-
 gel filtration Bacillus autolyticus
2.4.1.19 70000
-
-
 Geobacillus stearothermophilus
2.4.1.19 70000
-
 SDS-PAGE Bacillus autolyticus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Bacillus autolyticus
-
 11149
-
2.4.1.19 Bacillus autolyticus 11149
-
 11149
-
2.4.1.19 Bacillus sp. (in: Bacteria)
-
 AL-6
-
2.4.1.19 Bacillus sp. (in: Bacteria) AL-6
-
 AL-6
-
2.4.1.19 Geobacillus stearothermophilus
-
-
-
2.4.1.19 Klebsiella oxytoca
-
 formerly Klebsiella pneumoniae M5a1
-
2.4.1.19 Niallia circulans
-
 B. sp. var. alkalophilus No. 38-2, ATCC 21783
-
2.4.1.19 Paenibacillus macerans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19
-
 Geobacillus stearothermophilus
2.4.1.19
-
 Bacillus sp. (in: Bacteria)
2.4.1.19
-
 Paenibacillus macerans
2.4.1.19
-
 Bacillus autolyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.19 additional information
-
-
 Bacillus sp. (in: Bacteria)
2.4.1.19 additional information
-
-
 Niallia circulans
2.4.1.19 additional information
-
-
 Paenibacillus macerans
2.4.1.19 additional information
-
 332.5 units/mg, 1 unit is the amount of enzyme which decreases 10% of the absorbance at 700 nm per min Bacillus autolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 additional information
-
 Geobacillus stearothermophilus ?
-
 ?
2.4.1.19 additional information
-
 Niallia circulans ?
-
 ?
2.4.1.19 additional information
-
 Paenibacillus macerans ?
-
 ?
2.4.1.19 additional information sorbitol, mannitol, xylulose, galactose, fructose, lactose, glycerol and arabinose are not acceptors Bacillus autolyticus ?
-
 ?
2.4.1.19 additional information sorbitol, mannitol, xylulose, galactose, fructose, lactose, glycerol and arabinose are not acceptors Bacillus autolyticus 11149 ?
-
 ?
2.4.1.19 soluble starch + D-glucose
-
 Geobacillus stearothermophilus cyclodextrins
-
 r
2.4.1.19 soluble starch + D-glucose
-
 Paenibacillus macerans cyclodextrins
-
 ?
2.4.1.19 soluble starch + D-glucose
-
 Bacillus autolyticus cyclodextrins
-
 r
2.4.1.19 soluble starch + D-glucose
-
 Bacillus autolyticus 11149 cyclodextrins
-
 r
2.4.1.19 soluble starch + D-maltose
-
 Geobacillus stearothermophilus cyclodextrins
-
 r
2.4.1.19 soluble starch + D-maltose
-
 Niallia circulans cyclodextrins
-
 r
2.4.1.19 soluble starch + D-maltose
-
 Paenibacillus macerans cyclodextrins
-
 r
2.4.1.19 soluble starch + D-maltose
-
 Bacillus autolyticus cyclodextrins
-
 r
2.4.1.19 soluble starch + D-maltose
-
 Bacillus autolyticus 11149 cyclodextrins
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) Schardinger beta-dextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor ATCC 21783 Niallia circulans Schardinger beta-dextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) AL-6 Schardinger beta-dextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) beta-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor
-
 Niallia circulans beta-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor
-
 Paenibacillus macerans beta-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor potato starch, small amounts of alpha- and gamma-cyclodextrin Bacillus autolyticus beta-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) AL-6 beta-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor potato starch, small amounts of alpha- and gamma-cyclodextrin Bacillus autolyticus 11149 beta-cyclodextrin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.19 monomer
-
 Bacillus sp. (in: Bacteria)
2.4.1.19 monomer
-
 Niallia circulans
2.4.1.19 monomer
-
 Paenibacillus macerans
2.4.1.19 monomer 1 * 70000, SDS-PAGE Bacillus autolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.19 60
-
-
 Geobacillus stearothermophilus
2.4.1.19 60
-
-
 Bacillus sp. (in: Bacteria)
2.4.1.19 60
-
-
 Niallia circulans
2.4.1.19 60
-
-
 Paenibacillus macerans
2.4.1.19 60
-
-
 Bacillus autolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.19 5 6
-
 Bacillus autolyticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.19 5.5 9
-
 Geobacillus stearothermophilus
2.4.1.19 5.5 9
-
 Niallia circulans
2.4.1.19 5.5 9 stable for 12 h at 40°C, below 40°C stable for 1 h at pH 6.0 Bacillus autolyticus