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Literature summary extracted from

  • Ivony, K.; Szajani, B.; Seres, G.
    Immobilization of starch-degrading enzymes. I. A comparative study on soluble and immobilized cyclodextrin glycosyltransferase (1983), J. Appl. Biochem., 5, 158-164.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.4.1.19 synthesis application of immobilized enzyme is practically important for continous production of cyclodextrins, broad activity maximum is advantageous for industrial operations, immobilized enzyme is able to work at maximum efficiency at lower temperatures Paenibacillus macerans

General Stability

EC Number General Stability Organism
2.4.1.19 immobilization in strongly hydrophilic microenvironment markedly enhances conformational stability in a wide temperature and pH range Paenibacillus macerans

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Paenibacillus macerans
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.19 additional information
-
 specific activity after dissolution and dialysis 28 units/mg protein, 1 unit is the amount of enzyme which gives a linear increase of 1% transmission per minute at 40°C, immobilized enzyme 230-450 units/g solid Paenibacillus macerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 glycogen + acceptor
-
 Paenibacillus macerans beta-cyclodextrin
-
 r
2.4.1.19 maltooligosaccharides + glycosyl acceptor
-
 Paenibacillus macerans cyclodextrins
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Paenibacillus macerans cyclodextrins
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.4.1.19 Akrilex C cyclodextrin glycosyltransferase immobilized enzyme Paenibacillus macerans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.19 40 60 immobilized enzyme Paenibacillus macerans
2.4.1.19 60
-
-
 Paenibacillus macerans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.19 40 50 immobilized enzyme is stable at 40°C, heat inactivation above 50°C Paenibacillus macerans
2.4.1.19 50 70 immobilized enzyme is more stable than soluble enzyme, inactivation of the soluble enzyme at 50°C is 1.6 times, at 60°C is 6.9 times and at 70°C is 24.3 times faster Paenibacillus macerans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.19 5.5
-
 immobilized enzyme Paenibacillus macerans
2.4.1.19 5.9
-
-
 Paenibacillus macerans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.19 4 8.5
-
 Paenibacillus macerans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.19 4 10
-
 Paenibacillus macerans