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Literature summary extracted from

  • Lopez-Munguia, A.; Pelenc, V.; Remaud, M.; Biton, J.; Michel, J.M.; Lang, C.; Paul, F.; Monsan, P.
    Production and purification of alternansucrase, a glucosyltransferase from Leuconostoc mesenteroides NRRL B-1355, for the synthesis of oligoalternan (1993), Enzyme Microb. Technol., 15, 77-85.
No PubMed abstract available

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.140 Leuconostoc mesenteroides
-
 NRRL B-1355
-
2.4.1.140 Leuconostoc mesenteroides NRRL B-1355
-
 NRRL B-1355
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.140 NRLL B-1355 Leuconostoc mesenteroides

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.140 culture medium
-
 Leuconostoc mesenteroides
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.140 sucrose + alpha-D-glucan
-
 Leuconostoc mesenteroides alternating-1,6-1,3-alpha-D-glucan
-
 ?
2.4.1.140 sucrose + alpha-D-glucan
-
 Leuconostoc mesenteroides NRRL B-1355 alternating-1,6-1,3-alpha-D-glucan
-
 ?
2.4.1.140 sucrose + isomaltose
-
 Leuconostoc mesenteroides oligoalternan
-
 ?
2.4.1.140 sucrose + isomaltose
-
 Leuconostoc mesenteroides NRRL B-1355 oligoalternan
-
 ?
2.4.1.140 sucrose + maltose
-
 Leuconostoc mesenteroides oligoalternan panose is the first acceptor product ?
2.4.1.140 sucrose + maltose
-
 Leuconostoc mesenteroides NRRL B-1355 oligoalternan panose is the first acceptor product ?
2.4.1.140 sucrose + methyl-alpha-D-glucoside
-
 Leuconostoc mesenteroides oligoalternan
-
 ?
2.4.1.140 sucrose + methyl-alpha-D-glucoside
-
 Leuconostoc mesenteroides NRRL B-1355 oligoalternan
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.140 40
-
-
 Leuconostoc mesenteroides

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.140 30 50 30°C: about 65% of maximal activity, 50°C: about 55% of maximal activity Leuconostoc mesenteroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.140 40
-
 pH 5.4, half-life: 2 days Leuconostoc mesenteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.140 5.6
-
-
 Leuconostoc mesenteroides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.140 4.6 6.4 about 50% of activity maximum at pH 4.6 and 6.4 Leuconostoc mesenteroides