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Literature summary extracted from

  • Nakagawa, J.; Tamaki, S.; Tomioka, S.; Matsuhashi, M.
    Functional biosynthesis of cell wall peptidoglycan by polymorphic bifunctional polypeptides. Penicillin-binding protein 1Bs of Escherichia coli with activities of transglycosylase and transpeptidase (1984), J. Biol. Chem., 259, 13937-13946.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.129 benzylpenicillin stimulation, in the presence of 15% methanol, not at higher methanol concentrations or in the presence of deoxycholate Escherichia coli
2.4.1.129 Dimethylsulfoxide activation, in the absence of methanol, inhibits in the presence of 0.05% sarkosyl Escherichia coli
2.4.1.129 EDTA stimulation in the presence of high concentrations of methanol and detergents Escherichia coli
2.4.1.129 Imipenem stimulation, in the presence of 15% methanol, not at higher methanol concentrations or in the presence of deoxycholate Escherichia coli
2.4.1.129 additional information no activation by cephalexin, nocardicin A or mecillinam Escherichia coli
2.4.1.129 Sarkosyl activation Escherichia coli
2.4.1.129 Sodium 1,2-cyclohexanediamine-N,N,N',N'-tetraacetic acid stimulation in the presence of high concentrations of methanol and detergents Escherichia coli
2.4.1.129 sodium deoxycholate 0.05-0.1%, activation Escherichia coli
2.4.1.129 Triton X-100 activation, 0.05% Escherichia coli
2.4.1.129 Triton X-100 inhibits at 0.1% Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.129 Escherichia coli structural gene mrcB, recloned from plasmid pLC19-19 to high copy number plasmid pBr322, yielding plasmid pTM13 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.129 Ca2+
-
 Escherichia coli
2.4.1.129 Co2+
-
 Escherichia coli
2.4.1.129 Dimethylsulfoxide in the presence of 0.05% N-lauroylsarcosine Escherichia coli
2.4.1.129 EDTA in the absence of detergents, stimulates in the presence of high concentrations of methanol and detergents Escherichia coli
2.4.1.129 enramycin
-
 Escherichia coli
2.4.1.129 Fe2+
-
 Escherichia coli
2.4.1.129 macarbomycin
-
 Escherichia coli
2.4.1.129 Mn2+
-
 Escherichia coli
2.4.1.129 Moenomycin
-
 Escherichia coli
2.4.1.129 Ni2+
-
 Escherichia coli
2.4.1.129 Sodium 1,2-cyclohexanediamine-N,N,N',N'-tetraacetic acid in the absence of detergents, stimulates in the presence of high concentrations of methanol and detergents Escherichia coli
2.4.1.129 sodium deoxycholate in the presence of methanol, inhibits at 0.5% Escherichia coli
2.4.1.129 Triton X-100 inhibits at 0.1% Escherichia coli
2.4.1.129 Vancomycin
-
 Escherichia coli
2.4.1.129 Zn2+
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.129 membrane
-
 Escherichia coli 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.129 Mg2+ slight stimulation Escherichia coli
2.4.1.129 additional information no divalent cation requirement Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.129 90000
-
 x * 90000, Escherichia coli penicillin binding protein 1B alpha, beta or gamma Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.129 Escherichia coli
-
-
-
2.4.1.129 Escherichia coli JA200/pLC19-19
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.129 3 isozymes Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.4.1.129 -80°C, in concentrated PEG 6000 solution, stable for several months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.129 [GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol + GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 Escherichia coli [GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-diphosphoundecaprenol + undecaprenyl diphosphate
-
 ?
2.4.1.129 [GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol + GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 Escherichia coli JA200/pLC19-19 [GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-diphosphoundecaprenol + undecaprenyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.129 ? x * 90000, Escherichia coli penicillin binding protein 1B alpha, beta or gamma Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.129 37
-
-
 Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.129 20 45 about 60% of maximal activity at 20°C and about half-maximal activity at 45°C Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.129 60
-
 up to 87% loss of activity within 10 min Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.129 7 9 broad, in the presence of 0.1% deoxycholate Escherichia coli
2.4.1.129 8.5
-
 Tris-HCl buffer without deoxycholate Escherichia coli