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Literature summary extracted from

  • Nakagawa, J.; Tamaki, S.; Tomioka, S.; Matsuhashi, M.
    Functional biosynthesis of cell wall peptidoglycan by polymorphic bifunctional polypeptides. Penicillin-binding protein 1Bs of Escherichia coli with activities of transglycosylase and transpeptidase (1984), J. Biol. Chem., 259, 13937-13946.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.99.28 benzylpenicillin stimulation, in the presence of 15% methanol, not at higher methanol concentrations or in the presence of deoxycholate Escherichia coli
2.4.99.28 Dimethylsulfoxide activation, in the absence of methanol, inhibits in the presence of 0.05% sarkosyl Escherichia coli
2.4.99.28 EDTA stimulation in the presence of high concentrations of methanol and detergents Escherichia coli
2.4.99.28 Imipenem stimulation, in the presence of 15% methanol, not at higher methanol concentrations or in the presence of deoxycholate Escherichia coli
2.4.99.28 additional information no activation by cephalexin, nocardicin A or mecillinam Escherichia coli
2.4.99.28 Sarkosyl activation Escherichia coli
2.4.99.28 Sodium 1,2-cyclohexanediamine-N,N,N',N'-tetraacetic acid stimulation in the presence of high concentrations of methanol and detergents Escherichia coli
2.4.99.28 sodium deoxycholate 0.05-0.1%, activation Escherichia coli
2.4.99.28 Triton X-100 activation, 0.05% Escherichia coli
2.4.99.28 Triton X-100 inhibits at 0.1% Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.99.28 Escherichia coli structural gene mrcB, recloned from plasmid pLC19-19 to high copy number plasmid pBr322, yielding plasmid pTM13 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.99.28 Ca2+
-
 Escherichia coli
2.4.99.28 Co2+
-
 Escherichia coli
2.4.99.28 Dimethylsulfoxide in the presence of 0.05% N-lauroylsarcosine Escherichia coli
2.4.99.28 EDTA in the absence of detergents, stimulates in the presence of high concentrations of methanol and detergents Escherichia coli
2.4.99.28 enramycin
-
 Escherichia coli
2.4.99.28 Fe2+
-
 Escherichia coli
2.4.99.28 macarbomycin
-
 Escherichia coli
2.4.99.28 Mn2+
-
 Escherichia coli
2.4.99.28 Moenomycin
-
 Escherichia coli
2.4.99.28 Ni2+
-
 Escherichia coli
2.4.99.28 Sodium 1,2-cyclohexanediamine-N,N,N',N'-tetraacetic acid in the absence of detergents, stimulates in the presence of high concentrations of methanol and detergents Escherichia coli
2.4.99.28 sodium deoxycholate in the presence of methanol, inhibits at 0.5% Escherichia coli
2.4.99.28 Triton X-100 inhibits at 0.1% Escherichia coli
2.4.99.28 Vancomycin
-
 Escherichia coli
2.4.99.28 Zn2+
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.99.28 membrane
-
 Escherichia coli 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.99.28 Mg2+ slight stimulation Escherichia coli
2.4.99.28 additional information no divalent cation requirement Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.99.28 90000
-
 x * 90000, Escherichia coli penicillin binding protein 1B alpha, beta or gamma Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.99.28 Escherichia coli
-
-
-
2.4.99.28 Escherichia coli JA200/pLC19-19
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.99.28 3 isozymes Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.4.99.28 -80°C, in concentrated PEG 6000 solution, stable for several months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.99.28 [GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol + GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 Escherichia coli [GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-diphosphoundecaprenol + undecaprenyl diphosphate
-
 ?
2.4.99.28 [GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol + GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 Escherichia coli JA200/pLC19-19 [GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-diphosphoundecaprenol + undecaprenyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.99.28 ? x * 90000, Escherichia coli penicillin binding protein 1B alpha, beta or gamma Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.99.28 37
-
-
 Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.99.28 20 45 about 60% of maximal activity at 20°C and about half-maximal activity at 45°C Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.99.28 60
-
 up to 87% loss of activity within 10 min Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.99.28 7 9 broad, in the presence of 0.1% deoxycholate Escherichia coli
2.4.99.28 8.5
-
 Tris-HCl buffer without deoxycholate Escherichia coli