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Literature summary extracted from
- Dolichyldiphosphoryloligosaccharide-protein oligosaccharyltransferase; solubilization, purification, and properties (1980), Proc. Natl. Acad. Sci. USA, 77, 3811-3815.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.99.18 | EDTA | - |
Gallus gallus |  |
| 2.4.99.18 | Mg2+ | above 3 mM | Gallus gallus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.4.99.18 | rough endoplasmic reticulum | - |
Gallus gallus | 5791 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.99.18 | Mg2+ | absolute requirement for a divalent cation, Mg2+ is 30% as effective as Mn2+ | Gallus gallus | |
| 2.4.99.18 | Mg2+ | inhibition above 3 mM | Gallus gallus | |
| 2.4.99.18 | Mg2+ | 30% as effective as Mn2+ | Gallus gallus | |
| 2.4.99.18 | Mn2+ | requirement | Gallus gallus | |
| 2.4.99.18 | Mn2+ | absolute requirement for a divalent cation, 3 mM Mn2+ | Gallus gallus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.99.18 | Gallus gallus | - |
hen | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.99.18 | - |
Gallus gallus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.4.99.18 | oviduct | - |
Gallus gallus | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 2.4.99.18 | 4°C, less than 20% loss of activity within 1 week | Gallus gallus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.99.18 | dolichyl diphosphooligosaccharide + protein L-asparagine | (Glc)n-(Man)x-GlcNAc2-diphosphoryldolichol as oligosaccharyl donor and tryptic peptide consisting of residues 29-59 from bovine alpha-lactalbumin as acceptor | Gallus gallus | dolichyl diphosphate + glycoprotein with the oligosaccharide chain attached by N-glycosyl-linkage to protein L-asparagine | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.99.18 | 7 | 7.5 | - |
Gallus gallus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.99.18 | 6 | 8 | pH 6.0: about 30% of maximal activity, pH 8.0: about 20% of maximal activity | Gallus gallus |
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