BRENDA - Enzyme Database

Properties of a soluble polyprenyl phosphate: UDP-D-glucose glucosyltransferase

Villemez, C.L.; Carlo, P.L.; J. Biol. Chem. 254, 4814-4819 (1979)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.117
Reducing agent
required
Acanthamoeba castellanii
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.117
EDTA
inhibition reversed by MgCl2
Acanthamoeba castellanii
2.4.1.117
UDP
competitive
Acanthamoeba castellanii
2.4.1.117
UMP
reversible
Acanthamoeba castellanii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.117
0.0045
-
dolichyl phosphate
-
Acanthamoeba castellanii
2.4.1.117
0.0091
-
UDP-glucose
-
Acanthamoeba castellanii
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.117
soluble
-
Acanthamoeba castellanii
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.117
Mg2+
divalent cation required, either Mg2+ or Mn2+, Mn2+ stimulates the enzyme to a greater extent than Mg2+ at low concentrations, Mg2+ stimulates to a greater extent than Mn2+ at high concentration, but each is maximally effective at a concentration around 8 mM
Acanthamoeba castellanii
2.4.1.117
Mn2+
divalent cation required, either Mg2+ or Mn2+, Mn2+ stimulates the enzyme to a greater extent than Mg2+ at low concentrations, Mg2+ stimulates to a greater extent than Mn2+ at high concentration, but each is maximally effective at a concentration around 8 mM
Acanthamoeba castellanii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.117
additional information
Acanthamoeba castellanii
synthesis of dolichyl D-glucosyl phosphate, a glucosyl donor in the formation of lipid-linked core oligosaccharides
?
-
-
-
2.4.1.117
UDP-glucose + dolichyl phosphate
Acanthamoeba castellanii
-
UDP + dolichyl beta-D-glucosyl phosphate
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.117
Acanthamoeba castellanii
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.117
cyst
-
Acanthamoeba castellanii
-
Storage Stability
EC Number
Storage Stability
Organism
2.4.1.117
-20C, indefinitely stable below
Acanthamoeba castellanii
2.4.1.117
4C, stable for several days
Acanthamoeba castellanii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.117
additional information
no substrate: GDP-D-glucose, UDP-D-glucuronic acid, UDP-N-acetyl-D-glucosamine, UDP-D-xylose
488480
Acanthamoeba castellanii
?
-
-
-
-
2.4.1.117
additional information
synthesis of dolichyl D-glucosyl phosphate, a glucosyl donor in the formation of lipid-linked core oligosaccharides
488480
Acanthamoeba castellanii
?
-
-
-
-
2.4.1.117
UDP-glucose + dolichyl phosphate
-
488480
Acanthamoeba castellanii
UDP + dolichyl beta-D-glucosyl phosphate
-
-
-
r
2.4.1.117
UDP-glucose + dolichyl phosphate
-
488480
Acanthamoeba castellanii
UDP + dolichyl beta-D-glucosyl phosphate
-
488480
Acanthamoeba castellanii
r
2.4.1.117
UDP-glucose + ficaprenyl phosphate
-
488480
Acanthamoeba castellanii
UDP + ficaprenyl beta-D-glucosyl phosphate
-
-
-
r
2.4.1.117
UDP-glucose + solanesyl phosphate
-
488480
Acanthamoeba castellanii
UDP + solanesyl beta-D-glucosyl phosphate
-
-
-
r
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.4.1.117
30
-
-
Acanthamoeba castellanii
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.4.1.117
30
-
2 h, 50% loss of activity
Acanthamoeba castellanii
2.4.1.117
52
-
10 min, complete loss of activity
Acanthamoeba castellanii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.117
7
-
-
Acanthamoeba castellanii
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.1.117
5.6
8.8
pH 5.6: 25% of maximum activity, pH 8.8: about 40% of maximum activity
Acanthamoeba castellanii
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.117
Reducing agent
required
Acanthamoeba castellanii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.117
EDTA
inhibition reversed by MgCl2
Acanthamoeba castellanii
2.4.1.117
UDP
competitive
Acanthamoeba castellanii
2.4.1.117
UMP
reversible
Acanthamoeba castellanii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.117
0.0045
-
dolichyl phosphate
-
Acanthamoeba castellanii
2.4.1.117
0.0091
-
UDP-glucose
-
Acanthamoeba castellanii
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.117
soluble
-
Acanthamoeba castellanii
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.117
Mg2+
divalent cation required, either Mg2+ or Mn2+, Mn2+ stimulates the enzyme to a greater extent than Mg2+ at low concentrations, Mg2+ stimulates to a greater extent than Mn2+ at high concentration, but each is maximally effective at a concentration around 8 mM
Acanthamoeba castellanii
2.4.1.117
Mn2+
divalent cation required, either Mg2+ or Mn2+, Mn2+ stimulates the enzyme to a greater extent than Mg2+ at low concentrations, Mg2+ stimulates to a greater extent than Mn2+ at high concentration, but each is maximally effective at a concentration around 8 mM
Acanthamoeba castellanii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.117
additional information
Acanthamoeba castellanii
synthesis of dolichyl D-glucosyl phosphate, a glucosyl donor in the formation of lipid-linked core oligosaccharides
?
-
-
-
2.4.1.117
UDP-glucose + dolichyl phosphate
Acanthamoeba castellanii
-
UDP + dolichyl beta-D-glucosyl phosphate
-
-
r
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.117
cyst
-
Acanthamoeba castellanii
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.4.1.117
-20C, indefinitely stable below
Acanthamoeba castellanii
2.4.1.117
4C, stable for several days
Acanthamoeba castellanii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.117
additional information
no substrate: GDP-D-glucose, UDP-D-glucuronic acid, UDP-N-acetyl-D-glucosamine, UDP-D-xylose
488480
Acanthamoeba castellanii
?
-
-
-
-
2.4.1.117
additional information
synthesis of dolichyl D-glucosyl phosphate, a glucosyl donor in the formation of lipid-linked core oligosaccharides
488480
Acanthamoeba castellanii
?
-
-
-
-
2.4.1.117
UDP-glucose + dolichyl phosphate
-
488480
Acanthamoeba castellanii
UDP + dolichyl beta-D-glucosyl phosphate
-
-
-
r
2.4.1.117
UDP-glucose + dolichyl phosphate
-
488480
Acanthamoeba castellanii
UDP + dolichyl beta-D-glucosyl phosphate
-
488480
Acanthamoeba castellanii
r
2.4.1.117
UDP-glucose + ficaprenyl phosphate
-
488480
Acanthamoeba castellanii
UDP + ficaprenyl beta-D-glucosyl phosphate
-
-
-
r
2.4.1.117
UDP-glucose + solanesyl phosphate
-
488480
Acanthamoeba castellanii
UDP + solanesyl beta-D-glucosyl phosphate
-
-
-
r
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.4.1.117
30
-
-
Acanthamoeba castellanii
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.4.1.117
30
-
2 h, 50% loss of activity
Acanthamoeba castellanii
2.4.1.117
52
-
10 min, complete loss of activity
Acanthamoeba castellanii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.117
7
-
-
Acanthamoeba castellanii
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.1.117
5.6
8.8
pH 5.6: 25% of maximum activity, pH 8.8: about 40% of maximum activity
Acanthamoeba castellanii