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Literature summary extracted from

  • Villemez, C.L.; Carlo, P.L.
    Properties of a soluble polyprenyl phosphate: UDP-D-glucose glucosyltransferase (1979), J. Biol. Chem., 254, 4814-4819.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.117 Reducing agent required Acanthamoeba castellanii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.117 EDTA inhibition reversed by MgCl2 Acanthamoeba castellanii
2.4.1.117 UDP competitive Acanthamoeba castellanii
2.4.1.117 UMP reversible Acanthamoeba castellanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.117 0.0045
-
 dolichyl phosphate
-
 Acanthamoeba castellanii
2.4.1.117 0.0091
-
 UDP-glucose
-
 Acanthamoeba castellanii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.117 soluble
-
 Acanthamoeba castellanii
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.117 Mg2+ divalent cation required, either Mg2+ or Mn2+, Mn2+ stimulates the enzyme to a greater extent than Mg2+ at low concentrations, Mg2+ stimulates to a greater extent than Mn2+ at high concentration, but each is maximally effective at a concentration around 8 mM Acanthamoeba castellanii
2.4.1.117 Mn2+ divalent cation required, either Mg2+ or Mn2+, Mn2+ stimulates the enzyme to a greater extent than Mg2+ at low concentrations, Mg2+ stimulates to a greater extent than Mn2+ at high concentration, but each is maximally effective at a concentration around 8 mM Acanthamoeba castellanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.117 additional information Acanthamoeba castellanii synthesis of dolichyl D-glucosyl phosphate, a glucosyl donor in the formation of lipid-linked core oligosaccharides ?
-
 ?
2.4.1.117 UDP-glucose + dolichyl phosphate Acanthamoeba castellanii
-
 UDP + dolichyl beta-D-glucosyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.117 Acanthamoeba castellanii
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.117 cyst
-
 Acanthamoeba castellanii
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.117 -20°C, indefinitely stable below Acanthamoeba castellanii
2.4.1.117 4°C, stable for several days Acanthamoeba castellanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.117 additional information no substrate: GDP-D-glucose, UDP-D-glucuronic acid, UDP-N-acetyl-D-glucosamine, UDP-D-xylose Acanthamoeba castellanii ?
-
 ?
2.4.1.117 additional information synthesis of dolichyl D-glucosyl phosphate, a glucosyl donor in the formation of lipid-linked core oligosaccharides Acanthamoeba castellanii ?
-
 ?
2.4.1.117 UDP-glucose + dolichyl phosphate
-
 Acanthamoeba castellanii UDP + dolichyl beta-D-glucosyl phosphate
-
 r
2.4.1.117 UDP-glucose + ficaprenyl phosphate
-
 Acanthamoeba castellanii UDP + ficaprenyl beta-D-glucosyl phosphate
-
 r
2.4.1.117 UDP-glucose + solanesyl phosphate
-
 Acanthamoeba castellanii UDP + solanesyl beta-D-glucosyl phosphate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.117 30
-
-
 Acanthamoeba castellanii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.117 30
-
 2 h, 50% loss of activity Acanthamoeba castellanii
2.4.1.117 52
-
 10 min, complete loss of activity Acanthamoeba castellanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.117 7
-
-
 Acanthamoeba castellanii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.117 5.6 8.8 pH 5.6: 25% of maximum activity, pH 8.8: about 40% of maximum activity Acanthamoeba castellanii