EC Number | General Stability | Organism |
---|---|---|
2.4.1.111 | addition of high molarity buffer, 200 mM Tris/HCl, or 2-mercaptoethanol, optimal concentration 42 mM, increases stability considerably | Picea abies |
2.4.1.111 | in dilute solutions addition of 10-20% glycerol or ethylene glycol increases stability | Picea abies |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.111 | coniferin | non-competitive, product inhibition | Picea abies | |
2.4.1.111 | EDTA | no inhibition | Picea abies | |
2.4.1.111 | PCMB | 0.1 mM, complete inhibition | Picea abies | |
2.4.1.111 | UDP | non-competitive, product inhibition | Picea abies |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.111 | 0.22 | - |
UDPglucose | - |
Picea abies | |
2.4.1.111 | 0.25 | - |
coniferyl alcohol | - |
Picea abies | |
2.4.1.111 | 0.58 | - |
sinapyl alcohol | - |
Picea abies | |
2.4.1.111 | 0.64 | - |
Vanillin | - |
Picea abies |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.111 | 44000 | - |
gel filtration | Picea abies |
2.4.1.111 | 50000 | - |
1 * 50000, SDS-PAGE | Picea abies |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.111 | UDPglucose + coniferyl alcohol | Picea abies | participates in lignification of spruce | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.111 | Picea abies | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.111 | - |
Picea abies |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.4.1.111 | UDP-glucose + coniferyl alcohol = UDP + coniferin | mechanism | Picea abies |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.111 | cambium | - |
Picea abies | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.111 | 2.436 | - |
- |
Picea abies |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.1.111 | -20°C, 200 mM Tris/HCl, pH 7.5, 15% glycerol, stable for several months | Picea abies |
2.4.1.111 | 0-2°C, several weeks | Picea abies |
2.4.1.111 | glycerol, 15%, stabilizes during storage | Picea abies |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.111 | additional information | TDPglucose can replace UDPglucose with 17% efficiency | Picea abies | ? | - |
? | |
2.4.1.111 | UDP-glucose + coniferyl alcohol | forward reaction is about six times faster than the reverse reaction | Picea abies | UDP + coniferin | - |
r | |
2.4.1.111 | UDP-glucose + coniferyl aldehyde | glucosylated at 63% the rate of coniferyl alcohol | Picea abies | UDP + coniferaldehyde-4-O-beta-D-glucopyranoside | - |
? | |
2.4.1.111 | UDP-glucose + sinapaldehyde | glucosylated at 48% the rate of coniferyl alcohol | Picea abies | UDP + sinapaldehyde-4-O-beta-D-glucopyranoside | - |
? | |
2.4.1.111 | UDP-glucose + sinapyl alcohol | glucosylated at 14% of the activity with coniferyl alcohol | Picea abies | UDP + syringin | - |
? | |
2.4.1.111 | UDP-glucose + vanillic acid | glucosylated at 14% of the activity with coniferyl alcohol | Picea abies | UDP + vanillic acid-4-O-beta-D-glucopyranoside | - |
? | |
2.4.1.111 | UDP-glucose + vanillin | - |
Picea abies | UDP + vanillin-4-O-beta-D-glucopyranoside | - |
? | |
2.4.1.111 | UDPglucose + coniferyl alcohol | participates in lignification of spruce | Picea abies | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.111 | monomer | 1 * 50000, SDS-PAGE | Picea abies |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.111 | 36 | - |
- |
Picea abies |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.111 | 7.5 | - |
Tris-HCl, 0.1-0.2 M, or phosphate buffer | Picea abies |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.111 | 5.5 | 8.2 | pH 5.5: about 50% of maximal activity, pH 8.2: about 70% of maximal activity, phosphate buffer | Picea abies |
2.4.1.111 | 6.5 | 8.2 | about half-maximal activity at pH 6.5 and 83% of maximal activity at pH 8.2, Tris buffer | Picea abies |