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Literature summary extracted from

  • Chen, W.; Unligil, U.M.; Rini, J.M.; Stanley, P.
    Independent Lec1A CHO glycosylation mutants arise from point mutations in N-acetylglucosaminyltransferase I that reduce affinity for both substrates. Molecular consequences based on the crystal structure of GlcNAc-TI (2001), Biochemistry, 40, 8765-8772.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.101 C907T point mutation in mutant cell line Lec1A.3E and Lec1A.5J, altering interactions important for stabilization of the structure, correction of the mutation by site-directed mutagenesis restores enzyme activity Cricetulus griseus
2.4.1.101 G634A point mutation in mutant cell line Lec1A.2C, disrution of DxD motif responsible for interaction with UDP-GlcNAc and Mn2+, correction of the mutation by site-directed mutagenesis restores enzyme activity Cricetulus griseus
2.4.1.101 additional information 1 difference to published sequence with accession number U65791: C1340 is A1340 Cricetulus griseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.101 0.17
-
 UDP-N-acetyl-D-glucosamine wild-type CHO Cricetulus griseus
2.4.1.101 0.26
-
 Man5GlcNAc2 wild-type CHO Cricetulus griseus
2.4.1.101 5.43
-
 Man5GlcNAc2 mutant Lec1A Cricetulus griseus
2.4.1.101 7.56
-
 UDP-N-acetyl-D-glucosamine mutant Lec1A Cricetulus griseus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.101 Cricetulus griseus P70680 1 difference to published sequence U65791: C1340 is A1340
-
2.4.1.101 Mesocricetus auratus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.101 UDP-N-acetyl-alpha-D-glucosamine + Man5GlcNAc2-[protein] = UDP + Man5GlcNAc3-[protein] active site Cricetulus griseus
a
2.4.1.101 UDP-N-acetyl-alpha-D-glucosamine + Man5GlcNAc2-[protein] = UDP + Man5GlcNAc3-[protein] R303 is important for enzyme activity, conserved throughout all species Cricetulus griseus
a
2.4.1.101 UDP-N-acetyl-alpha-D-glucosamine + Man5GlcNAc2-[protein] = UDP + Man5GlcNAc3-[protein] D212 is the central residue of the DxD motif, conserved throughout all species Cricetulus griseus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.101 CHO cell wild-type, Lec1 cells are deficient in enzyme activity, mutant Lec1A cells show weak enzyme activity, 2% compared to wild-type, and produce complex N-glycans Cricetulus griseus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.101 additional information
-
 wild-type and mutant cell lines Cricetulus griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.101 UDP-N-acetyl-D-glucosamine + Man5GlcNAc2
-
 Cricetulus griseus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.101 GlcNAc-T I
-
 Mesocricetus auratus
2.4.1.101 GlcNAc-T I
-
 Cricetulus griseus