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Literature summary extracted from

  • Oppenheimer, C.L.; Hill, R.L.
    Purification and characterization of a rabbit liver alpha 1-3 mannoside beta 1-2 N-acetylglucosaminyltransferase (1981), J. Biol. Chem., 256, 799-804.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.101 repeated freeze-thawing, stable to Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.101 EDTA 3 mM Oryctolagus cuniculus
2.4.1.101 additional information high ionic strength, e.g. 0.1 M NaCl Oryctolagus cuniculus
2.4.1.101 UDP
-
 Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.101 0.45
-
 alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosamine
-
 Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.101 Ba2+ no activation Oryctolagus cuniculus
2.4.1.101 Ca2+ slight activation Oryctolagus cuniculus
2.4.1.101 Cd2+ activation Oryctolagus cuniculus
2.4.1.101 Cd2+ 78% efficiency Oryctolagus cuniculus
2.4.1.101 Co2+ activation Oryctolagus cuniculus
2.4.1.101 Co2+ 91% efficiency Oryctolagus cuniculus
2.4.1.101 Fe2+ activation Oryctolagus cuniculus
2.4.1.101 Fe2+ slight Oryctolagus cuniculus
2.4.1.101 Mg2+ activation Oryctolagus cuniculus
2.4.1.101 Mg2+ 61% efficiency Oryctolagus cuniculus
2.4.1.101 Mn2+ requirement Oryctolagus cuniculus
2.4.1.101 additional information no activation by Cu2+ Oryctolagus cuniculus
2.4.1.101 additional information no activation by Hg2+ Oryctolagus cuniculus
2.4.1.101 Ni2+ activation Oryctolagus cuniculus
2.4.1.101 Ni2+ 34% efficiency Oryctolagus cuniculus
2.4.1.101 Zn2+ activation Oryctolagus cuniculus
2.4.1.101 Zn2+ 19% efficiency Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.101 additional information
-
 two MW-species Oryctolagus cuniculus
2.4.1.101 46000
-
 x * 58000 + x * 46000, SDS-PAGE Oryctolagus cuniculus
2.4.1.101 58000
-
 x * 58000 + x * 46000, SDS-PAGE Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.101 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.101
-
 Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.101 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.101 2.51
-
 purified enzyme Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
2.4.1.101 -20°C, 20% glycerol v/v, up to a year Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.101 additional information
-
 Oryctolagus cuniculus ?
-
 ?
2.4.1.101 UDP-N-acetyl-alpha-D-glucosamine +
-
 Oryctolagus cuniculus ?
-
 ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: H Oryctolagus cuniculus UDP + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: H ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,3-beta-D-mannosyl-1,4-N-acetyl-D-glucosamine
-
 Oryctolagus cuniculus UDP + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-beta-D-mannosyl-1,4-N-acetyl-D-glucosamine
-
 ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R substrate specificity Oryctolagus cuniculus UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: H ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: H Oryctolagus cuniculus UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R R: H ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + dehexoso-orosomucoid
-
 Oryctolagus cuniculus ?
-
 ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetylglucosamine-1,4-(fucose-1,6)-N-acetyl-glucosamine-Asn-peptide no activity Oryctolagus cuniculus UDP + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetylglucosamine-1,4-(fucose-1,6)-N-acetyl-glucosamine-Asn-peptide
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.101 ? x * 58000 + x * 46000, SDS-PAGE Oryctolagus cuniculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.101 37
-
 assay at Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.101 6.3
-
-
 Oryctolagus cuniculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.101 5.8 6.7
-
 Oryctolagus cuniculus