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Literature summary extracted from

  • Nidetzky, B.; Griessler, R.; Weinhausel, A.; Haltrich, D.; Kulbe, K.D.
    Reaction engineering aspects of alpha-1,4-D-glucan phosphorylase catalysis: comparison of plant and bacterial enzymes for the continuous synthesis of D-glucose-1-phosphate (1997), Appl. Biochem. Biotechnol., 63-65, 159-172.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.1 unstable in the absence of phosphate, half-life at 4°C: less than 12 h Solanum tuberosum
2.4.1.1 unstable in the absence of phosphate, half-life at 4°C: less than 12 h Corynebacterium callunae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 additional information
-
 additional information 12 mg/ml maltodextrin Corynebacterium callunae
2.4.1.1 additional information
-
 additional information 1.3 mg/ml, maltodextrin Solanum tuberosum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Corynebacterium callunae
-
 DSM 20147
-
2.4.1.1 Solanum tuberosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1
-
 Solanum tuberosum
2.4.1.1
-
 Corynebacterium callunae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 6.5
-
-
 Corynebacterium callunae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.1 50 55
-
 Solanum tuberosum
2.4.1.1 50 55
-
 Corynebacterium callunae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.1 30
-
 half-life: 9 d in the presence of 300 mM phosphate Corynebacterium callunae
2.4.1.1 30
-
 half-life: 13 d in the presence of 300 mM phosphate Solanum tuberosum
2.4.1.1 45
-
 half-life: 20 d in the presence of 600 mM phosphate Solanum tuberosum
2.4.1.1 45
-
 half-life: 18 d in the presence of 600 mM phosphate Corynebacterium callunae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 7.8 8
-
 Solanum tuberosum