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Literature summary extracted from

  • Yonezawa, S.; Hori, S.H.
    Studies on phosphorylase isozymes in lower vertebrates. Purification and properties of lamprey phosphorylase (1977), Arch. Biochem. Biophys., 181, 447-453.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.1 5'-AMP activation Lethenteron camtschaticum
2.4.1.1 Na2SO4 500 mM, 40% activation of phosphorylase b in the presence of AMP Lethenteron camtschaticum
2.4.1.1 phosphate activation Lethenteron camtschaticum
2.4.1.1 phosphate in the presence of ATP-Mg Lethenteron camtschaticum
2.4.1.1 phosphate converts phosphorylase b into phosphorylase a Lethenteron camtschaticum
2.4.1.1 Phosphorylase kinase activation Lethenteron camtschaticum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 additional information
-
 additional information kinetic data Lethenteron camtschaticum
2.4.1.1 additional information
-
 additional information comparison of muscle phosphorylases of various animals Lethenteron camtschaticum
2.4.1.1 0.1
-
 AMP phosphorylase b Lethenteron camtschaticum
2.4.1.1 3.5
-
 alpha-D-glucose 1-phosphate phosphorylase a Lethenteron camtschaticum
2.4.1.1 15
-
 alpha-D-glucose 1-phosphate phosphorylase b, pH 6.3 Lethenteron camtschaticum
2.4.1.1 22
-
 alpha-D-glucose 1-phosphate phosphorylase b, pH 6.8 Lethenteron camtschaticum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 94000
-
 2 * 94000, SDS-PAGE Lethenteron camtschaticum
2.4.1.1 170000
-
 native PAGE Lethenteron camtschaticum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Lethenteron camtschaticum
-
 lamprey
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.1 side-chain modification activation by rabbit muscle calcium-activated phosphorylase kinase in the presence of ATP and Mg2+ Lethenteron camtschaticum
2.4.1.1 side-chain modification phosphorylation converts inactive phosphorylase b into active phosphorylase a Lethenteron camtschaticum

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 pH 5.4, ammonium sulfate, DEAE-cellulose, 5'-AMP-Sepharose Lethenteron camtschaticum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.1 additional information tissue distribution Lethenteron camtschaticum
-
2.4.1.1 muscle skeletal muscle Lethenteron camtschaticum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 60
-
 phosphorylase a, in the absence of AMP Lethenteron camtschaticum
2.4.1.1 75
-
 phosphorylase a, in the presence of 1 mM AMP Lethenteron camtschaticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Lethenteron camtschaticum [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.1 dimer 2 * 94000, SDS-PAGE Lethenteron camtschaticum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 6.3
-
-
 Lethenteron camtschaticum