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Literature summary extracted from

  • Kruger, N.J.; Ap Rees, T.
    Properties of alpha-glucan phosphorylase from pea chloroplasts (1983), Phytochemistry, 22, 1891-1898.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 ADPglucose 4 mM, 60% inhibition of chloroplast phosphorylase Pisum sativum
2.4.1.1 glucose 1-phosphate
-
 Pisum sativum
2.4.1.1 additional information not inhibited by fructose 6-phosphate; not inhibited by fructose, sucrose, dihydroxyacetone phosphate, 6-phosphogluconate, 2-phosphoglycollate, 2-phosphoglycerate, pyruvate Pisum sativum
2.4.1.1 phosphate
-
 Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 additional information
-
 additional information kinetic data Pisum sativum
2.4.1.1 0.45
-
 phosphate phosphorolysis at pH 7.2 Pisum sativum
2.4.1.1 0.96
-
 glucose 1-phosphate synthesis at pH 7.2 Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.1 chloroplast
-
 Pisum sativum 9507
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Pisum sativum
-
 pea, cv. Kelvedon Wonder
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 DEAE-cellulose, BioGel P-150, partial purification Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.1 leaf
-
 Pisum sativum
-
2.4.1.1 shoot
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 0.007
-
-
 Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 (dextrin)n-1 + glucose 1-phosphate
-
 Pisum sativum (dextrin)n + phosphate
-
 r
2.4.1.1 amylopectin + glucose 1-phosphate
-
 Pisum sativum amylopectin + phosphate
-
 r
2.4.1.1 amylose + phosphate
-
 Pisum sativum amylose + glucose 1-phosphate
-
 ?
2.4.1.1 glycogen + glucose 1-phosphate
-
 Pisum sativum glycogen + phosphate
-
 r
2.4.1.1 starch + glucose 1-phosphate soluble starch Pisum sativum starch + phosphate
-
 ?
2.4.1.1 starch + glucose 1-phosphate native chloroplast grains Pisum sativum starch + phosphate
-
 ?
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate no activity with pullulan Pisum sativum [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 5.6
-
 below, synthesis Pisum sativum
2.4.1.1 7.2
-
 phosphorolysis, 50% activity at pH 8.0 Pisum sativum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.1 4.36
-
 phosphate at pH 7.2 Pisum sativum
2.4.1.1 5.09
-
 glucose 1-phosphate at pH 7.2 Pisum sativum