EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.1 | phosphate | activation | Saccharomyces cerevisiae | |
2.4.1.1 | phosphate | phosphate content correlates with activity | Saccharomyces cerevisiae | |
2.4.1.1 | phosphate | 0.1-0.74 mol covalently bound phosphate per mol subunit | Saccharomyces cerevisiae | |
2.4.1.1 | Phosphorylase kinase | activation | Saccharomyces cerevisiae | |
2.4.1.1 | Phosphorylase kinase | incorporates 1 mol phosphate per mol subunit | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.1 | Phosphorylase phosphatase | reactivation by phosphorylase kinase | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.1 | additional information | - |
additional information | - |
Saccharomyces cerevisiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 100000 | - |
2 * 100000, SDS-PAGE | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.1 | Saccharomyces cerevisiae | - |
bottom fermenting brewer's yeast | - |
2.4.1.1 | Saccharomyces pastorianus | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.4.1.1 | side-chain modification | activation of enzyme by phosphorylase kinase, inactivation by phosphorylase phosphatase is due to phosphorylation of a single threonine residue in the N-terminus of the large subunit | Saccharomyces cerevisiae |
2.4.1.1 | side-chain modification | phosphate-content correlates with activity | Saccharomyces cerevisiae |
2.4.1.1 | side-chain modification | 0.1-0.74 mol covalently bound phosphate per mol subunit | Saccharomyces cerevisiae |
2.4.1.1 | side-chain modification | activation by phosphorylation | Saccharomyces cerevisiae |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.1 | Streptomycin, ammonium sulfate, DEAE-Sephadex, Sephadex G-200 | Saccharomyces cerevisiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.1 | 47.5 | - |
- |
Saccharomyces cerevisiae |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.1.1 | 4°C, 20-25 mg/ml in 100 mM succinate buffer, 0.02% NaN3, 2-3 months, little loss of activity | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.1 | [(1->4)-alpha-D-glucosyl]n + phosphate | - |
Saccharomyces pastorianus | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
2.4.1.1 | [(1->4)-alpha-D-glucosyl]n + phosphate | - |
Saccharomyces cerevisiae | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.1 | dimer | 2 * 100000, SDS-PAGE | Saccharomyces cerevisiae |
2.4.1.1 | dimer | presumably s- and l-subunit of very slight differences in molecular weight, less active: tetramer of l-subunit, inactive: octamer and larger oligomers | Saccharomyces cerevisiae |
2.4.1.1 | More | - |
Saccharomyces pastorianus |
2.4.1.1 | More | inactive enzyme tends to form larger oligomers | Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.1 | pyridoxal 5'-phosphate | requirement | Saccharomyces pastorianus | |
2.4.1.1 | pyridoxal 5'-phosphate | requirement | Saccharomyces cerevisiae |