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Literature summary extracted from

  • Gottschalk, G.
    Partial purification and some properties of the (R)-citrate synthase from Clostridium acidi-urici (1969), Eur. J. Biochem., 7, 301-106.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.3.3 2-mercaptoethanol 10-20 mM, reducing reagent is required for maximal activity Clostridium acidi-urici
2.3.3.3 glutathione reducing reagent is required for maximal activity Clostridium acidi-urici
2.3.3.3 L-cysteine reducing reagent is required for maximal activity Clostridium acidi-urici

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.3 Mn2+ 0.1 mM, required for maximal activity Clostridium acidi-urici

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.3 Clostridium acidi-urici
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.3.3.3 reducing reagents and anaerobic conditions are required for maximal activity Clostridium acidi-urici

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.3 isoelectric precipitation, DEAE-cellulose, DEAE-Sephadex Clostridium acidi-urici

Storage Stability

EC Number Storage Stability Organism
2.3.3.3 refrigerator, 24 h, 10% loss of activity Clostridium acidi-urici

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.3 acetyl-CoA + H2O + oxaloacetate stereospecific for (R)-citrates Clostridium acidi-urici citrate + CoA
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.3 8 8.5 sharp decrease in activity above Clostridium acidi-urici

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.3.3 6.5 8.5 approx. 50% activity at pH 7.0 Clostridium acidi-urici