Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Miziorko, H.M.
    3-Hydroxy-3-methylglutaryl-CoA synthase from chicken liver (1988), Methods Enzymol., 110, 19-26.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.10 3-carboxy-2,2,5,5,-tetramethyl-1-pyrrolidinyloxyl-CoA
-
 Gallus gallus
2.3.3.10 acetoacetyl-CoA
-
 Gallus gallus
2.3.3.10 DL-3-hydroxy-3-methylglutaryl-CoA competitive inhibition Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.10 0.00035
-
 acetoacetyl-CoA
-
 Gallus gallus
2.3.3.10 0.019
-
 S-(3-oxobutyl)-CoA
-
 Gallus gallus
2.3.3.10 0.1
-
 acetyl-CoA
-
 Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.10 mitochondrion
-
 Gallus gallus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.10 53000
-
 2 * 53000, SDS-PAGE Gallus gallus
2.3.3.10 105000
-
 sedimentation equlibrium Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.10 Gallus gallus
-
 chicken
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.10
-
 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.3.10 liver
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.10 0.85
-
-
 Gallus gallus

Storage Stability

EC Number Storage Stability Organism
2.3.3.10 -85°C, in presence of 30% glycerol stable for over 1 year Gallus gallus
2.3.3.10 4°C, in presence of 30% glycerol stable for several days Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.10 3-oxobutyryl-CoA + acetoacetyl-CoA + H2O
-
 Gallus gallus 3-hydroxy-3-methyl-5-oxoheptanedioyl-CoA + CoA
-
 ?
2.3.3.10 acetyl-3'-dephospho-CoA + acetoacetyl-CoA + H2O
-
 Gallus gallus 3-hydroxy-3-methylglutaryl-CoA + 3'-dephospho-CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
2.3.3.10 acetyl-CoA + N-acetyl-S-acetoacetylcysteamine + H2O
-
 Gallus gallus ? + CoA
-
 ?
2.3.3.10 H2O + acetyl-CoA + S-(3-oxobutyl)-CoA
-
 Gallus gallus CoA + S-(4-carboxy-3-hydroxy-isoamyl)-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.3.10 dimer 2 * 53000, SDS-PAGE Gallus gallus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.10 0.01
-
 acetoacetyl-CoA
-
 Gallus gallus
2.3.3.10 0.012
-
 DL-3-hydroxy-3-methylglutaryl-CoA
-
 Gallus gallus