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Literature summary extracted from

  • Robinson, M.S.; Danson, M.J.; Weitzman, P.D.
    Citrate synthase from a gram-positive bacterium (1983), Biochem. J., 213, 53-59.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.3.3.16 very instable without glycerol Priestia megaterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.16 ADP 10 mM, 64% inhibition; 64% inhibition at 10 mM Priestia megaterium
2.3.3.16 AMP 10 mM, 36% inhibition; 36% inhibition at 10 mM Priestia megaterium
2.3.3.16 ATP 10 mM, 79% inhibition; 79% inhibition at 10 mM Priestia megaterium
2.3.3.16 NAD+ 10 mM, 9% inhibition Priestia megaterium
2.3.3.16 NADH 10 mM, 31% inhibition; 1-10 mM; non-specific, 31% inhibition at 10 mM Priestia megaterium
2.3.3.16 NADP+ 10 mM, 32% inhibition Priestia megaterium
2.3.3.16 NADPH 10 mM, 57% inhibition; 57% inhibition at 10 mM Priestia megaterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.16 0.012
-
 oxaloacetate
-
 Priestia megaterium
2.3.3.16 0.076
-
 acetyl-CoA
-
 Priestia megaterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.16 40300
-
 2 * 40300, SDS-PAGE, gel filtration in guanidine-HCl, amino acid composition Priestia megaterium
2.3.3.16 40300
-
 2 * 40300, SDS-PAGE, gel filtration in guanidinium chloride Priestia megaterium
2.3.3.16 84000
-
 gel filtration Priestia megaterium
2.3.3.16 84000
-
 Stokes radius, sedimentation coefficient Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Priestia megaterium
-
-
-
2.3.3.16 Priestia megaterium D101
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.16
-
 Priestia megaterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.16 21.6
-
-
 Priestia megaterium
2.3.3.16 21.6
-
 purified enzyme Priestia megaterium

Storage Stability

EC Number Storage Stability Organism
2.3.3.16 4°C, pure citrate synthase is completely stable for at least 6 months Priestia megaterium
2.3.3.16 4°C, Tris-HCl buffer, pH 8.0, 20% v/v glycerol and 0.1 M KCl, at least 6 months Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Priestia megaterium citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Priestia megaterium D101 citrate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.16 dimer 2 * 40300, SDS-PAGE, gel filtration in guanidine-HCl, amino acid composition Priestia megaterium
2.3.3.16 dimer 2 * 40300, SDS-PAGE, gel filtration in guanidinium chloride Priestia megaterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.16 25
-
 enzyme assay at Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.16 8
-
 enzyme assay at Priestia megaterium