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Literature summary extracted from

  • Kato, M.; Nozawa, Y.
    Complete purification of arginyl-tRNA:protein arginyltransferase from hog kidney and production of its antibody (1984), Anal. Biochem., 143, 361-367.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.2.8 phosphate, 0.04 M, stabilizes Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.8 additional information antibodies to hog enzyme prepared in rabbit Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.8 cytoplasm soluble Sus scrofa 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.8 35000
-
 x * 35000, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.8 L-arginyl-tRNAArg + acceptor protein Sus scrofa
-
 tRNAArg + L-arginyl-[acceptor protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.8 Sus scrofa
-
 hog
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.8 affinity chromatography on heparin-Sepharose and angiotensin II-Sepharose combined with affinity elution with lactalbumin Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.8 kidney
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.8 7.6
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.3.2.8 4°C, concentrated enzyme solution, 0.5 M phosphate-KOH-buffer, pH 7.8, 0.1 M 2-mercaptoethanol, up to 6 months Sus scrofa
2.3.2.8 4°C, purified, 0.05 M Tris-HCl buffer, pH 8.0, 0.1 M 2-mercaptoethanol, 0.09 M KCl, 0.02 M spermine, 1 week Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.8 L-arginyl-tRNAArg + acceptor protein
-
 Sus scrofa tRNAArg + L-arginyl-[acceptor protein]
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.8 ? x * 35000, SDS-PAGE Sus scrofa