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Literature summary extracted from

  • Soffer, R.L.
    Enzymatic modification of proteins. II. Purification and properties of the arginyl transfer ribonucleic acid-protein transferase from rabbit liver cytoplasm (1970), J. Biol. Chem., 245, 731-737.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.2.8 2-mercaptoethanol requirement, 0.1 M, stabilizes the enzyme in a reduced state Oryctolagus cuniculus
2.3.2.8 dithiothreitol requirement, 0.01 M stabilizes the enzyme in a reduced state Oryctolagus cuniculus

General Stability

EC Number General Stability Organism
2.3.2.8 2-mercaptoethanol or dithiothreitol stabilize during storage Oryctolagus cuniculus
2.3.2.8 glycerol, 20%, stabilizes concentrated enzyme solutions during storage Oryctolagus cuniculus
2.3.2.8 polyethylene glycol, 30%, stabilizes concentrated solutions during storage Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.8 cytoplasm soluble Oryctolagus cuniculus 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.8 K+ requirement, optimal concentration is 0.2 M Oryctolagus cuniculus
2.3.2.8 K+ requirement for monovalent cations Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.8 L-arginyl-tRNAArg + acceptor protein Oryctolagus cuniculus
-
 tRNAArg + L-arginyl-[acceptor protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.8 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.8 7000fold Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.8 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.8 0.114
-
-
 Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
2.3.2.8 0°C, concentrated enzyme solution, 20% glycerol, t1/2: about 1 week Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.8 L-arginyl-tRNAArg + acceptor protein
-
 Oryctolagus cuniculus tRNAArg + L-arginyl-[acceptor protein]
-
 ?
2.3.2.8 L-arginyl-tRNAArg + albumin from bovine serum, accepts 1 mol arginine/mol Oryctolagus cuniculus tRNAArg + L-arginyl-albumin
-
 ?
2.3.2.8 L-arginyl-tRNAArg + fructose diphosphatase from rabbit liver Oryctolagus cuniculus tRNAArg + L-Arg-[fructose diphosphatase]
-
 ?
2.3.2.8 L-arginyl-tRNAArg + insulin less effective Oryctolagus cuniculus tRNAArg + L-arginyl-[insulin]
-
 ?
2.3.2.8 L-arginyl-tRNAArg + thyroglobulin accepts 2 mol arginine/mol Oryctolagus cuniculus tRNAArg + L-arginyl-[thyroglobulin]
-
 ?
2.3.2.8 L-arginyl-tRNAArg + transaldolase I and III from Candida Oryctolagus cuniculus tRNAArg + L-arginyl-[transaldolase]
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.8 37
-
 assay at Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.8 8.6 9.4
-
 Oryctolagus cuniculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.2.8 8 9.8 about half-maximal activity at pH 8.0, about 80% of maximal activity at pH 9.8 Oryctolagus cuniculus