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Literature summary extracted from

  • Mehdi, K.; Thierie, J.; Penninckx, M.J.
    gamma-Glutamyl transpeptidase in the yeast Saccharomyces cerevisiae and its role in the vacuolar transport and metabolism of glutathione (2001), Biochem. J., 359, 631-637.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.2.2 additional information nitrogen starvation induces enzyme activity Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 18
-
 glutathione
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.2 membrane bound to vascular membrane Saccharomyces cerevisiae 16020
-
2.3.2.2 vacuole membrane-bound Saccharomyces cerevisiae 5773
-
2.3.2.2 vacuole regulation of glutathione import into the vacuoles, kinetics Saccharomyces cerevisiae 5773
-
3.4.19.13 vacuole
-
 Saccharomyces cerevisiae 5773
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.2 glutathione + an amino acid Saccharomyces cerevisiae initial step of glutathione degradation L-cysteinylglycine + a 5-L-glutamyl-amino acid
-
 ?
2.3.2.2 glutathione + H2O Saccharomyces cerevisiae
-
 L-cysteinylglycine + L-glutamate
-
 ?
2.3.2.2 additional information Saccharomyces cerevisiae enzyme activates the yeast cadmium factor 1, responsible for glutathione import into the vacuole ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Saccharomyces cerevisiae
-
 wild-type and enzyme-deficient strain, nitrogen starvation-induced
-
3.4.19.13 Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.2.2 glycoprotein
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+
-
 Saccharomyces cerevisiae peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 glutathione + an amino acid initial step of glutathione degradation Saccharomyces cerevisiae L-cysteinylglycine + a 5-L-glutamyl-amino acid
-
 ?
2.3.2.2 glutathione + H2O
-
 Saccharomyces cerevisiae L-cysteinylglycine + L-glutamate
-
 ?
2.3.2.2 glutathione + H2O hydrolase reaction, concurrent to (auto-)transpeptidation Saccharomyces cerevisiae L-cysteinylglycine + L-glutamate
-
 ?
2.3.2.2 additional information enzyme activates the yeast cadmium factor 1, responsible for glutathione import into the vacuole Saccharomyces cerevisiae ?
-
 ?
3.4.19.13 additional information enzyme additionally catalyzes the reaction of EC 2.3.2.2, gamma-glutamyltransferase Saccharomyces cerevisiae ?
-
 ?

General Information

EC Number General Information Comment Organism
3.4.19.13 physiological function enzyme gamma-glutamyl transpeptidase and a L-Cys-Gly dipeptidase catalyse the complete hydrolysis of glutathione stored in the central vacuole of the yeast cell, prior to release of its constitutive amino acids L-glutamate, L-cysteine and glycine into the cytoplasm Saccharomyces cerevisiae