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Literature summary extracted from

  • Chu, L.; Xu, X.; Dong, Z.; Cappelli, D.; Ebersole, J.L.
    Role for recombinant gamma-glutamyltransferase from Treponema denticola in glutathione metabolism (2003), Infect. Immun., 71, 335-342.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.2.2 2-mercaptoethanol activation Treponema denticola
2.3.2.2 dithiothreitol activation Treponema denticola

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.2 expression in Escherichia coli Treponema denticola

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.2 additional information not affected by benzamidine Treponema denticola
2.3.2.2 Nalpha-4-tosyl-L-lysine chloromethyl ketone inactivation Treponema denticola
2.3.2.2 PMSF no effect Treponema denticola
2.3.2.2 Pronase inactivation Treponema denticola
2.3.2.2 proteinase K inactivation Treponema denticola

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.2 additional information
-
 amino acid composition Treponema denticola
2.3.2.2 additional information
-
 amino acid sequence Treponema denticola
2.3.2.2 31000
-
 x * 31000, SDS-PAGE Treponema denticola

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ Treponema denticola
-
 peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 glutathione + an amino acid Treponema denticola initial step of glutathione degradation L-cysteinylglycine + a 5-L-glutamyl-amino acid
-
 ?
2.3.2.2 additional information Treponema denticola purified recombinant enzyme added to Porphyromonas gingivalis results in H2S, ammonia and pyruvate release in this bacterium ?
-
 ?
2.3.2.2 additional information Treponema denticola L-Glu-4-nitroanilide competes with glutathione and inhibits production of H2S, ammonia and pyruvate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Treponema denticola
-
 strain ATCC 35405
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2 recombinant from E. coli Treponema denticola

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 158.4
-
 purified enzyme Treponema denticola

Storage Stability

EC Number Storage Stability Organism
2.3.2.2 -20°C, stable Treponema denticola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+
-
 Treponema denticola peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ L-cysteine is no substrate Treponema denticola peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 5-D-glutamyl-4-nitroanilide + acceptor
-
 Treponema denticola 4-nitroaniline + 5-D-glutamyl-acceptor
-
 ?
2.3.2.2 glutathione + an amino acid initial step of glutathione degradation Treponema denticola L-cysteinylglycine + a 5-L-glutamyl-amino acid
-
 ?
2.3.2.2 glutathione + H2O hydrolase reaction, concurrent to (auto-)transpeptidation Treponema denticola L-cysteinylglycine + L-glutamate
-
 ?
2.3.2.2 additional information purified recombinant enzyme added to Porphyromonas gingivalis results in H2S, ammonia and pyruvate release in this bacterium Treponema denticola ?
-
 ?
2.3.2.2 additional information L-Glu-4-nitroanilide competes with glutathione and inhibits production of H2S, ammonia and pyruvate Treponema denticola ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.2 ? x * 31000, SDS-PAGE Treponema denticola

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.2.2 56
-
 inactivation Treponema denticola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.2 additional information
-
 pI: 5.9 Treponema denticola