Literature summary extracted from

Sachdev, G.P.; Leahy, D.S.; Chace, K.V.
Phenobarbital and related compounds as novel inhibitors of gamma-glutamyltranspeptidase (1983), Biochim. Biophys. Acta, 749, 125-129.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.3.2.2	hexobarbital	-	Rattus norvegicus
2.3.2.2	Phenobarbital	active site-directed, irreversible inactivation, optimal at pH 9.0, serine/borate or GSH protects, maleate protects slightly, kinetics	Rattus norvegicus
2.3.2.2	Thiobarbituric acid	active site-directed, irreversible inactivation, optimal at pH 9.5, serine/borate or GSH protects, maleate protects slightly, kinetics	Rattus norvegicus

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.3.2.2	Rattus norvegicus	-	male, Sprague-Dawley	-

Reaction

EC Number	Reaction	Comment	Organism	Reaction ID
2.3.2.2	a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid	active site on small subunit	Rattus norvegicus	a

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
2.3.2.2	kidney	-	Rattus norvegicus	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.3.2.2	(5-L-glutamyl)-peptide + acceptor + H+	-	Rattus norvegicus	peptide + 5-L-glutamyl amino acid	-	?
2.3.2.2	5-L-glutamyl-4-nitroanilide + glycylglycine	-	Rattus norvegicus	4-nitroaniline + 5-L-glutamylglycylglycine	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.3.2.2	More	small subunit MW: 20000	Rattus norvegicus
2.3.2.2	More	the active site resides on the small subunit	Rattus norvegicus

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.3.2.2	37	-	assay at	Rattus norvegicus

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
2.3.2.2	20	-	Thiobarbituric acid	-	Rattus norvegicus
2.3.2.2	30	-	hexobarbital	-	Rattus norvegicus
2.3.2.2	43	-	Phenobarbital	-	Rattus norvegicus

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Release 2023.1 (January 2023)