BRENDA - Enzyme Database

Purification and some properties of gamma-glutamyl transpeptidase from azo dye-induced hepatoma

Taniguchi, N.; J. Biochem. 75, 473-480 (1974) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.2.2
5,5'-dithiobis(2-nitrobenzoate)
weak
Rattus norvegicus
2.3.2.2
EDTA
no inhibition
Rattus norvegicus
2.3.2.2
iodoacetamide
no inhibition
Rattus norvegicus
2.3.2.2
iodoacetate
no inhibition
Rattus norvegicus
2.3.2.2
N-ethylmaleimide
weak
Rattus norvegicus
2.3.2.2
p-chloromercuribenzoate
no inhibition
Rattus norvegicus
2.3.2.2
Zn2+
strong
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.2.2
1.1
-
L-Glu-4-nitroanilide
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.2.2
membrane
-
Rattus norvegicus
16020
-
2.3.2.2
microsome
-
Rattus norvegicus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.2.2
Ca2+
slight activation
Rattus norvegicus
2.3.2.2
Ca2+
activation, transpeptidase
Rattus norvegicus
2.3.2.2
Ca2+
not hydrolase
Rattus norvegicus
2.3.2.2
Mg2+
slight activation
Rattus norvegicus
2.3.2.2
Mg2+
activation, transpeptidase
Rattus norvegicus
2.3.2.2
Mg2+
not hydrolase
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.2.2
additional information
-
amino acid composition
Rattus norvegicus
2.3.2.2
113000
-
analytical ultracentrifugation
Rattus norvegicus
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.3.2.2
Rattus norvegicus
-
male, Donryu
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
2.3.2.2
glycoprotein
36% carbohydrate
Rattus norvegicus
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.3.2.2
solubilized by deoxycholate
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.2.2
hepatoma cell
azo dye-induced
Rattus norvegicus
-
2.3.2.2
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.2.2
181
-
purified enzyme
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
2.3.2.2
-22°C, at least 3 months
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
Glu-donor is 5-L-Glu-4-nitroanilide
487913
Rattus norvegicus
peptide + 5-L-glutamyl amino acid
-
-
-
ir
2.3.2.2
glutathione + acceptor
-
487913
Rattus norvegicus
cysteinylglycine + 5-L-glutamyl-acceptor
-
487913
Rattus norvegicus
?
2.3.2.2
L-Glu-4-nitroanilide + glutathione
-
487913
Rattus norvegicus
4-nitroaniline + 5-L-glutamyl-glutathione
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.2.2
37
-
assay at
Rattus norvegicus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.3.2.2
58
-
12 min
Rattus norvegicus
2.3.2.2
58
-
in the presence of glutathione, 0.02 M, at least 60 min stable
Rattus norvegicus
2.3.2.2
58
-
20% of initial activity retained after 60 min
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.2.2
additional information
-
pI: 3.0
Rattus norvegicus
2.3.2.2
8.8
9
-
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.2.2
8.1
9.6
about 70% of maximal activity at pH 8.1, about 75% of maximal activity at pH 9.6
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.2.2
5,5'-dithiobis(2-nitrobenzoate)
weak
Rattus norvegicus
2.3.2.2
EDTA
no inhibition
Rattus norvegicus
2.3.2.2
iodoacetamide
no inhibition
Rattus norvegicus
2.3.2.2
iodoacetate
no inhibition
Rattus norvegicus
2.3.2.2
N-ethylmaleimide
weak
Rattus norvegicus
2.3.2.2
p-chloromercuribenzoate
no inhibition
Rattus norvegicus
2.3.2.2
Zn2+
strong
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.2.2
1.1
-
L-Glu-4-nitroanilide
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.2.2
membrane
-
Rattus norvegicus
16020
-
2.3.2.2
microsome
-
Rattus norvegicus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.2.2
Ca2+
slight activation
Rattus norvegicus
2.3.2.2
Ca2+
activation, transpeptidase
Rattus norvegicus
2.3.2.2
Ca2+
not hydrolase
Rattus norvegicus
2.3.2.2
Mg2+
slight activation
Rattus norvegicus
2.3.2.2
Mg2+
activation, transpeptidase
Rattus norvegicus
2.3.2.2
Mg2+
not hydrolase
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.2.2
additional information
-
amino acid composition
Rattus norvegicus
2.3.2.2
113000
-
analytical ultracentrifugation
Rattus norvegicus
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
2.3.2.2
glycoprotein
36% carbohydrate
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.2.2
solubilized by deoxycholate
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.2.2
hepatoma cell
azo dye-induced
Rattus norvegicus
-
2.3.2.2
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.2.2
181
-
purified enzyme
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.2.2
-22°C, at least 3 months
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.3.2.2
(5-L-glutamyl)-peptide + acceptor + H+
Glu-donor is 5-L-Glu-4-nitroanilide
487913
Rattus norvegicus
peptide + 5-L-glutamyl amino acid
-
-
-
ir
2.3.2.2
glutathione + acceptor
-
487913
Rattus norvegicus
cysteinylglycine + 5-L-glutamyl-acceptor
-
487913
Rattus norvegicus
?
2.3.2.2
L-Glu-4-nitroanilide + glutathione
-
487913
Rattus norvegicus
4-nitroaniline + 5-L-glutamyl-glutathione
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.2.2
37
-
assay at
Rattus norvegicus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.3.2.2
58
-
12 min
Rattus norvegicus
2.3.2.2
58
-
in the presence of glutathione, 0.02 M, at least 60 min stable
Rattus norvegicus
2.3.2.2
58
-
20% of initial activity retained after 60 min
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.2.2
additional information
-
pI: 3.0
Rattus norvegicus
2.3.2.2
8.8
9
-
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.2.2
8.1
9.6
about 70% of maximal activity at pH 8.1, about 75% of maximal activity at pH 9.6
Rattus norvegicus