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Literature summary extracted from

  • Zhang, L.; Jackson-Machelski, E.; Gordon, J.I.
    Biochemical studies of Saccharomyces cerevisiae myristoyl-coenzyme A:protein N-myristoyltransferase mutants (1996), J. Biol. Chem., 271, 33131-33140.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.97 expression of His-tagged wild-type and mutants in Escherichia coli Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.97 A202T site-directed mutagenesis, alterations of both peptide and myristoyl binding sites, 3 to 6fold increased Ki for S-(2-oxo)-pentadecyl-CoA and 6 to 9fold increased Ki for SC-58272 Saccharomyces cerevisiae
2.3.1.97 C217R site-directed mutagenesis, 3 to 6fold increase in Ki for inhibitor SC-58272, selective alteration of the enzymes peptide binding site Saccharomyces cerevisiae
2.3.1.97 D417V site-directed mutagenesis, reduced activity, temperature-sensitive Saccharomyces cerevisiae
2.3.1.97 F413S site-directed mutagenesis, reduced activity, temperature-sensitive Saccharomyces cerevisiae
2.3.1.97 K389I site-directed mutagenesis, reduced activity, temperature-sensitive Saccharomyces cerevisiae
2.3.1.97 L171S site-directed mutagenesis, reduced activity, temperature-sensitive Saccharomyces cerevisiae
2.3.1.97 L408S site-directed mutagenesis, reduced activity, temperature-sensitive Saccharomyces cerevisiae
2.3.1.97 L420S site-directed mutagenesis, reduced activity, temperature-sensitive Saccharomyces cerevisiae
2.3.1.97 N102T site-directed mutagenesis, reduced activity, temperature-sensitive Saccharomyces cerevisiae
2.3.1.97 N426I site-directed mutagenesis, mutation of myristoyl-binding site Saccharomyces cerevisiae
2.3.1.97 S328P site-directed mutagenesis, highly reduced activity Saccharomyces cerevisiae
2.3.1.97 V395D site-directed mutagenesis, reduced activity, temperature-sensitive Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.97 S-(2-Oxo)-pentadecyl-CoA competitive Saccharomyces cerevisiae
2.3.1.97 SC-58272 peptidomimetic derived from the N-terminal sequence of the substrate ADP-ribosylation factor-2, i.e. Arf2p Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.97 myristoyl-CoA + glycylpeptide Saccharomyces cerevisiae enzyme is essential for vegetative growth N-myristoylglycylpeptide + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.97 Saccharomyces cerevisiae
-
 gene nmt1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.97 His-tagged recombinant wild-type and mutants from Escherichia coli Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.97 tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein] ordered bi bi mechanism Saccharomyces cerevisiae
a
2.3.1.97 tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein] peptide binding site Saccharomyces cerevisiae
a
2.3.1.97 tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein] myristoyl-CoA binding site Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.97 myristoyl-CoA + glycylpeptide specific for Gly at N-terminus Saccharomyces cerevisiae N-myristoylglycylpeptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + glycylpeptide enzyme is essential for vegetative growth Saccharomyces cerevisiae N-myristoylglycylpeptide + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.97 Nmt1p
-
 Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.97 additional information
-
 additional information Ki of several mutants for S-(2-oxo)-pentadecyl-CoA at 24 and 37°C Saccharomyces cerevisiae
2.3.1.97 0.000005
-
 S-(2-Oxo)-pentadecyl-CoA wild-type, 37°C Saccharomyces cerevisiae
2.3.1.97 0.000009
-
 SC-58272 His-tagged wild-type, 37°C Saccharomyces cerevisiae
2.3.1.97 0.000014
-
 SC-58272 wild-type, 37°C Saccharomyces cerevisiae
2.3.1.97 0.000055
-
 SC-58272 His-tagged mutant C217R, 37°C Saccharomyces cerevisiae
2.3.1.97 0.000082
-
 SC-58272 His-tagged mutant A202T, 37°C Saccharomyces cerevisiae