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Literature summary extracted from

  • Glover, C.J.; Felsted, R.L.
    Identification and characterization of multiple forms of bovine brain N-myristoyltransferase (1995), J. Biol. Chem., 270, 23226-23233.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.97 additional information
-
 enzyme forms multiple high molecular weight aggregates of fully active monomers Bos taurus
2.3.1.97 60000
-
 1 * 60000, SDS-PAGE Bos taurus
2.3.1.97 126000 390000 high molecular weight aggregates, gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.97 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.97 no glycoprotein
-
 Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.97 multiple forms from brain Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.97 brain
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
2.3.1.97 -15°C, conversion of 60000 Da form into 47000 Da form, loss of 30% activity within 10 days Bos taurus
2.3.1.97 4°C, conversion of 60000 Da form into 47000 Da form, activity is stable over several months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.97 myristoyl-CoA + Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Asp-Pro-Ser-Gln-Arg-Arg-Arg pp60src-derived peptide Bos taurus N-myristoyl-Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Asp-Pro-Ser-Gln-Arg-Arg-Arg + CoA
-
 ?
2.3.1.97 myristoyl-CoA + glycylpeptide specific for Gly at N-terminus Bos taurus N-myristoylglycylpeptide + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.97 monomer 1 * 60000, SDS-PAGE Bos taurus
2.3.1.97 monomer 1 * 47000-49000, active form after storage at 4°C, SDS-PAGE Bos taurus
2.3.1.97 More 2 interconvertable forms of 60000 and 47000 Da, both catalytically active, formation of multimeric complexes, molecular weight reduction is not only due to proteolysis but has a possible regulatory role in vivo Bos taurus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.97 NMT
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.97 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.97 7.6
-
 assay at Bos taurus