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Literature summary extracted from

  • Antonenkov, V.D.; Croes, K.; Waelkens, E.; Van Veldhoven, P.P.; Mannaerts, G.P.
    Identification, purification and characterization of an acetoacetyl-CoA thiolase from rat liver peroxisomes (2000), Eur. J. Biochem., 267, 2981-2990.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.9 0.008
-
 CoA peroxisomal thiolase, thiolysis Rattus norvegicus
2.3.1.9 0.009
-
 acetoacetyl-CoA peroxisomal thiolase, thiolysis Rattus norvegicus
2.3.1.9 0.016
-
 acetoacetyl-CoA cytosolic thiolase, thiolysis Rattus norvegicus
2.3.1.9 0.02
-
 CoA cytosolic thiolase, thiolysis Rattus norvegicus
2.3.1.9 0.035
-
 acetoacetyl-CoA peroxisomal thiolase, synthesis Rattus norvegicus
2.3.1.9 0.08
-
 acetoacetyl-CoA cytosolic thiolase, synthesis Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.9 peroxisome
-
 Rattus norvegicus 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.9 42000
-
 4 * 42000, peroxisomal thiolase, SDS-PAGE Rattus norvegicus
2.3.1.9 160000
-
 peroxisomal thiolase, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA Rattus norvegicus peroxisomal thiolase most probably catalyzes the first reaction in peroxisomal cholesterol and dolichol synthesis acetyl-CoA + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.9 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.9 peroxisomal thiolase, DEAE-cellulose, phosphocellulose column, Blue-Sepharose Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.9 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.9 100 130 peroxisomal thiolase, thiolysis Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA
-
 Rattus norvegicus acetyl-CoA + acetyl-CoA
-
 r
2.3.1.9 CoA + acetoacetyl-CoA peroxisomal thiolase most probably catalyzes the first reaction in peroxisomal cholesterol and dolichol synthesis Rattus norvegicus acetyl-CoA + acetyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.9 tetramer 4 * 42000, peroxisomal thiolase, SDS-PAGE Rattus norvegicus