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Literature summary extracted from

  • Duncombe, G.R.; Frerman, F.E.
    Molecular and catalytic properties of the acetoacetyl-coenzyme A thiolase of Escherichia coli (1976), Arch. Biochem. Biophys., 176, 159-170.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.9 acetoacetyl-CoA substrate inhibition above 0.105 mM Escherichia coli
2.3.1.9 iodoacetamide 2 mM, complete inactivation after approx. 60 min, 0.3 mM acetoacetyl-CoA or 1.6 mM acetyl-CoA protect Escherichia coli
2.3.1.9 N-ethylmaleimide
-
 Escherichia coli
2.3.1.9 p-chloromercuribenzoate
-
 Escherichia coli
2.3.1.9 Sodium borohydride
-
 Escherichia coli
2.3.1.9 sulfhydryl reagents
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.9 0.47
-
 acetyl-CoA synthesis Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.9 42000
-
 4 * 42000, SDS-PAGE Escherichia coli
2.3.1.9 156000
-
 sucrose density gradient sedimentation Escherichia coli
2.3.1.9 166000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA Escherichia coli
-
 acetyl-CoA + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.9 Escherichia coli
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.3.1.9 sensitive to borohydride reduction Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.9 1078
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.3.1.9 0°C, 0.05 M Tris-HCl, pH 7.8, 2 mM dithiothreitol, 1 mM EDTA, 20% glycerol, 6 weeks, 50% loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA
-
 Escherichia coli acetyl-CoA + acetyl-CoA
-
 ?
2.3.1.9 CoA + acetoacetyl-CoA
-
 Escherichia coli acetyl-CoA + acetyl-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.9 tetramer 4 * 42000, SDS-PAGE Escherichia coli