BRENDA - Enzyme Database show

The purification, properties and internal peptide sequences of alcohol acetyltransferase isolated from Saccharomyces cerevisiae Kyokai No. 7

Minetoki, T.; Bogaki, T.; Iwamatsu, A.; Fujii, T.; Hamachi, M.; Biosci. Biotechnol. Biochem. 57, 2094-2098 (1993)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.84
5,5'-dithiobis(2-nitrobenzoate)
complete inhibition
Saccharomyces cerevisiae
2.3.1.84
Cd2+
-
Saccharomyces cerevisiae
2.3.1.84
Cu2+
-
Saccharomyces cerevisiae
2.3.1.84
Hg2+
-
Saccharomyces cerevisiae
2.3.1.84
linoleic acid
2 mM, 86% inhibition
Saccharomyces cerevisiae
2.3.1.84
linolenic acid
2 mM, 68% inhibition
Saccharomyces cerevisiae
2.3.1.84
p-chloromercuribenzoate
complete inhibition
Saccharomyces cerevisiae
2.3.1.84
Zn2+
-
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.84
0.025
-
acetyl-CoA
strain NCYC 366
Saccharomyces cerevisiae
2.3.1.84
0.19
-
acetyl-CoA
-
Saccharomyces cerevisiae
2.3.1.84
29.8
-
isoamyl alcohol
-
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.84
22000
-
x * 22000, SDS-PAGE
Cladosporium cladosporioides
2.3.1.84
60000
-
x * 60000, SDS-PAGE
Saccharomyces cerevisiae
2.3.1.84
270000
-
gel filtration
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.84
Cladosporium cladosporioides
-
-
-
2.3.1.84
Saccharomyces cerevisiae
-
sake yeast strain Kyokai No. 7
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.84
PBE 94 column, DEAE-cellulose, Toypearl HW60, hydroxyapatite, octyl Sepharose
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.3.1.84
5.47
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.84
isoamylalcohol + acetyl-CoA
slightly higher activity with straight-chain alcohols than with branched-chain alcohols, activity with straight-chain alcohols in descending order: pentanol, butanol, propanol, very low activity with ethanol, activity with branched-chain alcohols in descending order of carbon number: C5, C4, C3
487559
Saccharomyces cerevisiae
isoamyl acetate + CoA
-
487559
Saccharomyces cerevisiae
-
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.84
?
x * 22000, SDS-PAGE
Cladosporium cladosporioides
2.3.1.84
?
x * 60000, SDS-PAGE
Saccharomyces cerevisiae
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.3.1.84
25
-
-
Saccharomyces cerevisiae
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.3.1.84
10
-
loss of 20% activity
Saccharomyces cerevisiae
2.3.1.84
20
-
rapid inactivation above
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.84
5.9
-
-
Cladosporium cladosporioides
2.3.1.84
8
-
-
Saccharomyces cerevisiae
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.3.1.84
7.5
8
no loss of activity after 22 h at 4C
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.84
5,5'-dithiobis(2-nitrobenzoate)
complete inhibition
Saccharomyces cerevisiae
2.3.1.84
Cd2+
-
Saccharomyces cerevisiae
2.3.1.84
Cu2+
-
Saccharomyces cerevisiae
2.3.1.84
Hg2+
-
Saccharomyces cerevisiae
2.3.1.84
linoleic acid
2 mM, 86% inhibition
Saccharomyces cerevisiae
2.3.1.84
linolenic acid
2 mM, 68% inhibition
Saccharomyces cerevisiae
2.3.1.84
p-chloromercuribenzoate
complete inhibition
Saccharomyces cerevisiae
2.3.1.84
Zn2+
-
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.84
0.025
-
acetyl-CoA
strain NCYC 366
Saccharomyces cerevisiae
2.3.1.84
0.19
-
acetyl-CoA
-
Saccharomyces cerevisiae
2.3.1.84
29.8
-
isoamyl alcohol
-
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.84
22000
-
x * 22000, SDS-PAGE
Cladosporium cladosporioides
2.3.1.84
60000
-
x * 60000, SDS-PAGE
Saccharomyces cerevisiae
2.3.1.84
270000
-
gel filtration
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.84
PBE 94 column, DEAE-cellulose, Toypearl HW60, hydroxyapatite, octyl Sepharose
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.3.1.84
5.47
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.84
isoamylalcohol + acetyl-CoA
slightly higher activity with straight-chain alcohols than with branched-chain alcohols, activity with straight-chain alcohols in descending order: pentanol, butanol, propanol, very low activity with ethanol, activity with branched-chain alcohols in descending order of carbon number: C5, C4, C3
487559
Saccharomyces cerevisiae
isoamyl acetate + CoA
-
487559
Saccharomyces cerevisiae
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.84
?
x * 22000, SDS-PAGE
Cladosporium cladosporioides
2.3.1.84
?
x * 60000, SDS-PAGE
Saccharomyces cerevisiae
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.3.1.84
25
-
-
Saccharomyces cerevisiae
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.3.1.84
10
-
loss of 20% activity
Saccharomyces cerevisiae
2.3.1.84
20
-
rapid inactivation above
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.84
5.9
-
-
Cladosporium cladosporioides
2.3.1.84
8
-
-
Saccharomyces cerevisiae
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.3.1.84
7.5
8
no loss of activity after 22 h at 4C
Saccharomyces cerevisiae