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Literature summary extracted from

  • Wolf, E.; Vassilev, A.; Makino, Y.; Sali, A.; Nakatani, Y.; Burley, S.K.
    Crystal structure of a GCN5-related N-acetyltransferase: Serratia marcescens aminoglycoside 3-N-acetyltransferase (1998), Cell, 94, 439-449.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.81 wild-type and truncated enzymes expressed in Escherichia coli Serratia marcescens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.81 X-ray structure of a recombinant enzyme, expressed in its selenomethionine substituted form, cocrystallization with 5 mM CoA at 4°C and pH: 7.8, crystal cryoprotection achieved with 17% glycerol and 8% 2R,3R-butanediol, active center found by X-ray analysis and model building Serratia marcescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.81 soluble
-
 Serratia marcescens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.81 18630
-
 18649 Da predicted from nucleotide sequence, 18630 Da found by mass spectrometry Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.81 Serratia marcescens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.81 side-chain modification cleavage of the hexahistidine tag by tobacco etch virus protease Serratia marcescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.81 acetyl-CoA + gentamicin
-
 Serratia marcescens CoA + N3'-acetylgentamicin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.81 aminoglycoside 3-N-acetyltransferase
-
 Serratia marcescens