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Literature summary extracted from

  • Rado, T.A.; Hoch, J.A.
    Phosphotransacetylase from Bacillus subtilis: purification and physiological studies (1973), Biochim. Biophys. Acta, 321, 114-125.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.8 ADP inhibitory at 1 mM Bacillus subtilis
2.3.1.8 AMP inhibitory at 1 mM Bacillus subtilis
2.3.1.8 ATP inhibitory at 1 mM Bacillus subtilis
2.3.1.8 Ba2+ 15% inhibition at 1 mM, 76% inhibition at 10 mM Bacillus subtilis
2.3.1.8 Ca2+ 50% inhibition at 1 mM Bacillus subtilis
2.3.1.8 Mn2+ 50% inhibition at 1 mM Bacillus subtilis
2.3.1.8 palmitoyl-CoA competitive Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.8 0.058
-
 butyryl-CoA
-
 Bacillus subtilis
2.3.1.8 0.06
-
 acetyl-CoA
-
 Bacillus subtilis
2.3.1.8 0.15
-
 propionyl-CoA
-
 Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.8 K+ 3.4fold stimulation at 10 mM Bacillus subtilis
2.3.1.8 NH4+ 5.2fold stimulation at 10 mM Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.8 90000
-
 gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate Bacillus subtilis
-
 CoA + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.8 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.8 600fold Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.8 1371
-
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate
-
 Bacillus subtilis CoA + acetyl phosphate
-
 r
2.3.1.8 acetyl-CoA + phosphate formation of acetyl-CoA is favored Bacillus subtilis CoA + acetyl phosphate
-
 r
2.3.1.8 butyryl-CoA + phosphate
-
 Bacillus subtilis CoA + butyryl phosphate
-
 ?
2.3.1.8 propionyl-CoA + phosphate
-
 Bacillus subtilis CoA + propionyl phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.8 7.6
-
-
 Bacillus subtilis