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Literature summary extracted from

  • Bergmeyer, H.U.; Holz, G.; Klotsch, H.; Lang, G.
    Phosphotransacetylase aus Clostridium kluyveri (1963), Biochem. Z., 338, 114-121.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.8
-
 Clostridium kluyveri

General Stability

EC Number General Stability Organism
2.3.1.8 dilute solutions are instable, in frozen state stable Clostridium kluyveri

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.8 2,2'-dipyridyl
-
 Clostridium kluyveri
2.3.1.8 CuSO4
-
 Clostridium kluyveri
2.3.1.8 K+
-
 Clostridium kluyveri
2.3.1.8 KCN weak Clostridium kluyveri
2.3.1.8 Mg2+
-
 Clostridium kluyveri
2.3.1.8 MnCl2 weak Clostridium kluyveri
2.3.1.8 Na+
-
 Clostridium kluyveri
2.3.1.8 p-chloromercuribenzoate
-
 Clostridium kluyveri
2.3.1.8 Tris(hydroxymethyl)aminomethane weak Clostridium kluyveri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.8 0.56
-
 CoA
-
 Clostridium kluyveri
2.3.1.8 0.66
-
 acetyl phosphate
-
 Clostridium kluyveri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.8 NH4+ required for maximum activity, optimum at 7 mM (NH4)2SO4 Clostridium kluyveri
2.3.1.8 phosphate 1 mM, weak activation Clostridium kluyveri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.8 38000 41000 ultracentrifugation Clostridium kluyveri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate Clostridium kluyveri
-
 CoA + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.8 Clostridium kluyveri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.8
-
 Clostridium kluyveri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.8 9100
-
 after crystallization Clostridium kluyveri

Storage Stability

EC Number Storage Stability Organism
2.3.1.8 0-4°C, 2.7-3.0 M ammonium sulfate, months Clostridium kluyveri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate
-
 Clostridium kluyveri CoA + acetyl phosphate
-
 r
2.3.1.8 acetyl-CoA + phosphate in reverse reaction specific for CoA Clostridium kluyveri CoA + acetyl phosphate
-
 r

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.8 22 40 only small effect of temperature in this range Clostridium kluyveri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.8 40
-
 5 min, 1.5 mg/ml protein concentration, 0.15 M ammonium sulfate, pH 6, stable Clostridium kluyveri
2.3.1.8 45
-
 5 min, 1.5 mg/ml protein concentration, 0.15 M ammonium sulfate, pH 6, 15% loss of activity Clostridium kluyveri
2.3.1.8 50
-
 5 min, 1.5 mg/ml protein concentration, 0.15 M ammonium sulfate, pH 6, 48% loss of activity Clostridium kluyveri
2.3.1.8 60
-
 5 min, 1.5 mg/ml protein concentration, 0.15 M ammonium sulfate, pH 6, 98% loss of activity Clostridium kluyveri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.8 7.4
-
-
 Clostridium kluyveri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.8 6.6 8.2 about 50% of activity maximum at pH 6.6 pH and 8.2 Clostridium kluyveri

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.8 6
-
 1.5 mg/ml protein concentration, 0.15 M ammonium sulfate, 40°C, 5 min, stable Clostridium kluyveri