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Literature summary extracted from

  • Stadtman, E.R.
    Phosphotransacetylase from Clostridium kluyveri (1955), Methods Enzymol., 1, 596-599.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.3.1.8 activity is lost upon dialysis and cannot be restored by addition of known cofactors or crude boiled extracts Clostridium kluyveri

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.8 ADP
-
 Clostridium kluyveri
2.3.1.8 ATP
-
 Clostridium kluyveri
2.3.1.8 citrate buffer 0.1 M, pH 8.0 Clostridium kluyveri
2.3.1.8 Diethylbarbiturate potassium diethylbarbiturate buffer, 0.1 M, pH 8.0 Clostridium kluyveri
2.3.1.8 Li+
-
 Clostridium kluyveri
2.3.1.8 Na+
-
 Clostridium kluyveri
2.3.1.8 potassium diphosphate 0.1 M, pH 8.0 Clostridium kluyveri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.8 K+ enzyme is inactive in absence of K+ or NH4+, maximal activation at about 0.02 M Clostridium kluyveri
2.3.1.8 NH4+ K+ or NH4+ at concentration above 10 mM required for maximum activity, enzyme is inactive in absence of K+ or NH4+, maximal activation at about 0.02 M Clostridium kluyveri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate Clostridium kluyveri
-
 CoA + acetyl phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.8 Clostridium kluyveri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.8
-
 Clostridium kluyveri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.8 additional information
-
 assay methods Clostridium kluyveri

Storage Stability

EC Number Storage Stability Organism
2.3.1.8 -10°C, stable for several months Clostridium kluyveri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate
-
 Clostridium kluyveri CoA + acetyl phosphate
-
 ?
2.3.1.8 acetyl-CoA + phosphate in reverse reaction specific for CoA Clostridium kluyveri CoA + acetyl phosphate
-
 ?
2.3.1.8 arsenate + acetyl-CoA
-
 Clostridium kluyveri acetyl arsenate + CoA
-
 ?
2.3.1.8 butyryl-CoA + phosphate at a rate 0.01 as rapid as acetyl-CoA Clostridium kluyveri CoA + butyryl phosphate
-
 ?
2.3.1.8 additional information arsenolysis Clostridium kluyveri ?
-
 ?
2.3.1.8 propionyl-CoA + phosphate at a rate 0.1 to 0.5 as rapid as acetyl-CoA Clostridium kluyveri CoA + propionyl phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.8 7.4 8.4
-
 Clostridium kluyveri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.8 6.6 9
-
 Clostridium kluyveri