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Literature summary extracted from

  • Cronin, C.N.
    Redesign of choline acetyltransferase specificity by protein engineering (1998), J. Biol. Chem., 273, 24465-24469.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.6 N514R is described as ChAT-R, the introduction of an Arg at position 514 in rat enzyme is predicted to provide the ionic charge required to interact with, and neutralize, the carboxyl group of carnitine Rattus norvegicus
2.3.1.6 V459T/D460E/N461T is described as ChAT-TET Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.6 0.00201
-
 acetyl-CoA cosubstrate carnitine Rattus norvegicus
2.3.1.6 0.00284
-
 acetyl-CoA enzyme mutant ChAT-R/TET, cosubstrate carnitine Rattus norvegicus
2.3.1.6 0.012
-
 acetyl-CoA enzyme mutant ChAT-R, cosubstrate carnitine Rattus norvegicus
2.3.1.6 0.0148
-
 acetyl-CoA enzyme mutant ChAT-TET, cosubstrate carnitine Rattus norvegicus
2.3.1.6 0.015
-
 acetyl-CoA cosubstrate choline Rattus norvegicus
2.3.1.6 0.0155
-
 acetyl-CoA enzyme mutant ChAT-R, cosubstrate choline Rattus norvegicus
2.3.1.6 0.0225
-
 acetyl-CoA enzyme mutant ChAT-TET, cosubstrate choline Rattus norvegicus
2.3.1.6 0.0516
-
 acetyl-CoA enzyme mutant ChAT-R/TET, cosubstrate acetyl-CoA Rattus norvegicus
2.3.1.6 0.192
-
 choline cosubstrate acetyl-CoA Rattus norvegicus
2.3.1.6 1.88
-
 choline enzyme mutant ChAT-R, cosubstrate acetyl-CoA Rattus norvegicus
2.3.1.6 4.8
-
 carnitine enzyme mutant ChAT-R/TET, cosubstrate acetyl-CoA Rattus norvegicus
2.3.1.6 5.79
-
 choline enzyme mutant ChAT-TET, cosubstrate acetyl-CoA Rattus norvegicus
2.3.1.6 100
-
 carnitine cosubstrate acetyl-CoA Rattus norvegicus
2.3.1.6 100
-
 carnitine enzyme mutant ChAT-R, cosubstrate acetyl-CoA Rattus norvegicus
2.3.1.6 100
-
 carnitine enzyme mutant ChAT-TET, cosubstrate acetyl-CoA Rattus norvegicus
2.3.1.6 128
-
 choline enzyme mutant ChAT-TET, cosubstrate acetyl-CoA Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.6 Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.6 acetyl-CoA + carnitine
-
 Rattus norvegicus acetylcarnitine + CoA
-
 ?
2.3.1.6 acetyl-CoA + choline
-
 Rattus norvegicus acetylcholine + CoA
-
 r