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Literature summary extracted from

  • Maisonneuve, S.; Bessoule, J.J.; Lessire, R.; Delseny, M.; Roscoe, T.J.
    Mutagenesis of a plastidial lysophosphatidic acid acyltransferase (2000), Biochem. Soc. Trans., 28, 961-964.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.51 transformation of a modified plasmid into Escherichia coli JC201 enzyme-deficient strain and transformation of the plasmid DNA containing the cDNA BAT2 encoding the plastidial enzyme into the Escherichia coli XL-1 red mutator strain Brassica napus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.51 C247Y loss of the ability of the plastidial enzyme to restore growth to JC201, suggests that C247 is essential for activity Brassica napus
2.3.1.51 D199A the mutant fails to grow beyond 0.04 D600 after transfer to the non-permissive temperature. D199 is essential for the ability of the plastidial enzyme to complement the defective acyltransferase activity of JC201 Brassica napus
2.3.1.51 E266A the plastidial enzyme containing this mutation is unable to complement the temperature-sensitive phenotype of JC201, indicating an essential role for this acidic residue Brassica napus
2.3.1.51 G140S loss of the ability of the plastidial enzyme to restore growth to JC201, suggests that G140 is essential for activity Brassica napus
2.3.1.51 H194A the mutant fails to grow beyond 0.04 D600 after transfer to the non-permissive temperature. H194 is essential for the ability of the plastidial enzyme to complement the defective acyltransferase activity of JC201 Brassica napus
2.3.1.51 K84Q greatly impaired growth rate, possibly indicating an importance of the N-terminus of the mature protein in determining enzyme activity Brassica napus
2.3.1.51 L203P loss of the ability of the plastidial enzyme to restore growth to JC201, suggests that L203 is essential for activity Brassica napus
2.3.1.51 P187L loss of the ability of the plastidial enzyme to restore growth to JC201, suggests that P187 is essential for activity Brassica napus
2.3.1.51 R92G greatly impaired growth rate, possibly indicating an importance of the N-terminus of the mature protein in determining enzyme activity Brassica napus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.51 plastid
-
 Brassica napus 9536
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.51 Brassica napus Q9XFW4
-
-