BRENDA - Enzyme Database

Properties of acyl-coenzyme A:1-acylglycerophosphate acyltransferase and lipases in porcine erythrocyte membranes

Mizuno, M.; Sugiura, Y.; Okuyama, H.; J. Lipid Res. 25, 843-850 (1984)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.3.1.51
the long time required for fractionation after hypotonic hemolysis probably contributes to the loss of activity
Sus scrofa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.51
additional information
at low concentrations of enzyme protein, the enzyme is possibly inactivated by the detergent action of substrates
Sus scrofa
2.3.1.51
sodium deoxycholate
concentration-dependent inactivation
Sus scrofa
2.3.1.51
Triton X-100
concentration-dependent inactivation
Sus scrofa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.51
membrane
-
Homo sapiens
16020
-
2.3.1.51
membrane
-
Sus scrofa
16020
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.51
Homo sapiens
-
-
-
2.3.1.51
Sus scrofa
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.51
erythrocyte
mature
Homo sapiens
-
2.3.1.51
erythrocyte
mature
Sus scrofa
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487192
Homo sapiens
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
487192
Homo sapiens
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA esters of unsaturated 16- and 18-carbon fatty acids are the best substrates, followed by those saturated 14-, 16-, and 18-carbon acids, and the unsaturated 20-carbon acids
487192
Sus scrofa
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
487192
Sus scrofa
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
with 1 to 5 double bonds
487192
Sus scrofa
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
487192
Sus scrofa
?
2.3.1.51
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
487192
Sus scrofa
CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
-
487192
Sus scrofa
?
2.3.1.51
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
487192
Sus scrofa
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
-
487192
Sus scrofa
?
2.3.1.51
acyl-CoA + 2-acyl-sn-glycerol 3-phosphate
the enzyme does not acylate 2-acyl-sn-glycerol 3-phosphate
487192
Sus scrofa
CoA + ?
-
-
-
-
2.3.1.51
acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
-
487192
Sus scrofa
CoA + ?
-
487192
Sus scrofa
?
2.3.1.51
acyl-CoA + myristoyl-sn-glycerol 3-phosphate
-
487192
Sus scrofa
CoA + ?
-
487192
Sus scrofa
?
2.3.1.51
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487192
Sus scrofa
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
-
487192
Sus scrofa
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.51
22
-
assay at
Sus scrofa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.51
7.4
-
assay at
Sus scrofa
General Stability (protein specific)
EC Number
General Stability
Organism
2.3.1.51
the long time required for fractionation after hypotonic hemolysis probably contributes to the loss of activity
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.51
additional information
at low concentrations of enzyme protein, the enzyme is possibly inactivated by the detergent action of substrates
Sus scrofa
2.3.1.51
sodium deoxycholate
concentration-dependent inactivation
Sus scrofa
2.3.1.51
Triton X-100
concentration-dependent inactivation
Sus scrofa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.51
membrane
-
Homo sapiens
16020
-
2.3.1.51
membrane
-
Sus scrofa
16020
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.51
erythrocyte
mature
Homo sapiens
-
2.3.1.51
erythrocyte
mature
Sus scrofa
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487192
Homo sapiens
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
487192
Homo sapiens
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA esters of unsaturated 16- and 18-carbon fatty acids are the best substrates, followed by those saturated 14-, 16-, and 18-carbon acids, and the unsaturated 20-carbon acids
487192
Sus scrofa
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
487192
Sus scrofa
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
with 1 to 5 double bonds
487192
Sus scrofa
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
487192
Sus scrofa
?
2.3.1.51
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
487192
Sus scrofa
CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
-
487192
Sus scrofa
?
2.3.1.51
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
487192
Sus scrofa
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
-
487192
Sus scrofa
?
2.3.1.51
acyl-CoA + 2-acyl-sn-glycerol 3-phosphate
the enzyme does not acylate 2-acyl-sn-glycerol 3-phosphate
487192
Sus scrofa
CoA + ?
-
-
-
-
2.3.1.51
acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
-
487192
Sus scrofa
CoA + ?
-
487192
Sus scrofa
?
2.3.1.51
acyl-CoA + myristoyl-sn-glycerol 3-phosphate
-
487192
Sus scrofa
CoA + ?
-
487192
Sus scrofa
?
2.3.1.51
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487192
Sus scrofa
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
-
487192
Sus scrofa
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.51
22
-
assay at
Sus scrofa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.51
7.4
-
assay at
Sus scrofa