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Literature summary extracted from

  • Hares, W.; Frentzen, M.
    Substrate specificities of the membrane-bound and partially purified microsomal acyl-CoA:1-acylglycerol-3-phosphate acyltransferase from etiolated shoots of Pisum sativum (L.) (1991), Planta, 185, 124-131.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.51 CHAPS above 0.4% w/v Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.51 endoplasmic reticulum
-
 Pisum sativum 5783
-
2.3.1.51 microsome predominantly located in endoplasmic reticulum Pisum sativum
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate Pisum sativum involved in phospholipid biosynthesis CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 additional information Pisum sativum acyl-CoA thioesters can be considered as the physiological substrates of the microsomal enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.51 Pisum sativum
-
 pea, cv. Kleine Rheinlaenderin
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.51 partial Pisum sativum
2.3.1.51 using solubilization with CHAPS at high ionic strength, and anion-exchange chromatography on Mono Q column Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.51 shoot etiolated Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.51 0.0066
-
-
 Pisum sativum

Storage Stability

EC Number Storage Stability Organism
2.3.1.51 -20°C, particulate preparation, several days Pisum sativum
2.3.1.51 4°C, soluble preparation, at least 1 day Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate i.e. lysophosphatidic acid, highly specific for 1-acyl-sn-glycerol 3-phosphate, catalyzes acylation of position 2 hydroxyl-group Pisum sativum CoA + 1,2-diacyl-sn-glycerol 3-phosphate i.e. phosphatidic acid ?
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate unsaturated preferred Pisum sativum CoA + 1,2-diacyl-sn-glycerol 3-phosphate i.e. phosphatidic acid ?
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate pronounced specificities and selectivities for unsaturated C18-CoA thioesters Pisum sativum CoA + 1,2-diacyl-sn-glycerol 3-phosphate i.e. phosphatidic acid ?
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate involved in phospholipid biosynthesis Pisum sativum CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate 1-oleoyl-sn-glycerol 3-phosphate is the best acceptor Pisum sativum CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 Pisum sativum CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 acyl-CoA + 2-acyl-sn-glycerol 3-phosphate the enzyme does not acylate 2-acyl-sn-glycerol 3-phosphate Pisum sativum CoA + ?
-
 ?
2.3.1.51 acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
-
 Pisum sativum CoA + ?
-
 ?
2.3.1.51 acyl-CoA + myristoyl-sn-glycerol 3-phosphate
-
 Pisum sativum CoA + ?
-
 ?
2.3.1.51 gondoyl-CoA + 1-acyl-sn-glycerol 3-phosphate gondoyl-CoA is utilized less efficiently for the reaction than oleoyl-CoA Pisum sativum CoA + 1-acyl-2-gondoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 additional information the microsomal enzyme displays a specificity for unsaturated C18-CoA thioesters and this fatty-acid specificity is not altered in the course of the purification procedure Pisum sativum ?
-
 ?
2.3.1.51 additional information acyl-CoA thioesters can be considered as the physiological substrates of the microsomal enzyme Pisum sativum ?
-
 ?
2.3.1.51 oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate oleoyl-CoA is the best substrate, both membrane-bound and partially purified enzyme Pisum sativum CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 palmitoyl-ACP + 1-acyl-sn-glycerol 3-phosphate
-
 Pisum sativum ACP + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Pisum sativum CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.51 25
-
 assay at Pisum sativum