BRENDA - Enzyme Database

Substrate specificities of the membrane-bound and partially purified microsomal acyl-CoA:1-acylglycerol-3-phosphate acyltransferase from etiolated shoots of Pisum sativum (L.)

Hares, W.; Frentzen, M.; Planta 185, 124-131 (1991)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.51
CHAPS
above 0.4% w/v
Pisum sativum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.51
endoplasmic reticulum
-
Pisum sativum
5783
-
2.3.1.51
microsome
predominantly located in endoplasmic reticulum
Pisum sativum
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
Pisum sativum
involved in phospholipid biosynthesis
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
Pisum sativum
?
2.3.1.51
additional information
Pisum sativum
acyl-CoA thioesters can be considered as the physiological substrates of the microsomal enzyme
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.51
Pisum sativum
-
pea, cv. Kleine Rheinlaenderin
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.51
partial; using solubilization with CHAPS at high ionic strength, and anion-exchange chromatography on Mono Q column
Pisum sativum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.51
shoot
etiolated
Pisum sativum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.3.1.51
0.0066
-
-
Pisum sativum
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.51
-20C, particulate preparation, several days
Pisum sativum
2.3.1.51
4C, soluble preparation, at least 1 day
Pisum sativum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
i.e. lysophosphatidic acid, highly specific for 1-acyl-sn-glycerol 3-phosphate, catalyzes acylation of position 2 hydroxyl-group
487184
Pisum sativum
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
i.e. phosphatidic acid
487184
Pisum sativum
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
unsaturated preferred
487184
Pisum sativum
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
i.e. phosphatidic acid
487184
Pisum sativum
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
pronounced specificities and selectivities for unsaturated C18-CoA thioesters
487184
Pisum sativum
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
i.e. phosphatidic acid
487184
Pisum sativum
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
involved in phospholipid biosynthesis
487184
Pisum sativum
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
?
2.3.1.51
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
1-oleoyl-sn-glycerol 3-phosphate is the best acceptor
487184
Pisum sativum
CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
?
2.3.1.51
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
-
2.3.1.51
acyl-CoA + 2-acyl-sn-glycerol 3-phosphate
the enzyme does not acylate 2-acyl-sn-glycerol 3-phosphate
487184
Pisum sativum
CoA + ?
-
-
-
-
2.3.1.51
acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
CoA + ?
-
487184
Pisum sativum
-
2.3.1.51
acyl-CoA + myristoyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
CoA + ?
-
487184
Pisum sativum
-
2.3.1.51
gondoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
gondoyl-CoA is utilized less efficiently for the reaction than oleoyl-CoA
487184
Pisum sativum
CoA + 1-acyl-2-gondoyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
?
2.3.1.51
additional information
the microsomal enzyme displays a specificity for unsaturated C18-CoA thioesters and this fatty-acid specificity is not altered in the course of the purification procedure
487184
Pisum sativum
?
-
-
-
-
2.3.1.51
additional information
acyl-CoA thioesters can be considered as the physiological substrates of the microsomal enzyme
487184
Pisum sativum
?
-
-
-
-
2.3.1.51
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
oleoyl-CoA is the best substrate, both membrane-bound and partially purified enzyme
487184
Pisum sativum
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
?
2.3.1.51
palmitoyl-ACP + 1-acyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
ACP + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
?
2.3.1.51
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.3.1.51
25
-
assay at
Pisum sativum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.51
CHAPS
above 0.4% w/v
Pisum sativum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.51
endoplasmic reticulum
-
Pisum sativum
5783
-
2.3.1.51
microsome
predominantly located in endoplasmic reticulum
Pisum sativum
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
Pisum sativum
involved in phospholipid biosynthesis
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
Pisum sativum
?
2.3.1.51
additional information
Pisum sativum
acyl-CoA thioesters can be considered as the physiological substrates of the microsomal enzyme
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.51
partial; using solubilization with CHAPS at high ionic strength, and anion-exchange chromatography on Mono Q column
Pisum sativum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.51
shoot
etiolated
Pisum sativum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.3.1.51
0.0066
-
-
Pisum sativum
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.51
-20C, particulate preparation, several days
Pisum sativum
2.3.1.51
4C, soluble preparation, at least 1 day
Pisum sativum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
i.e. lysophosphatidic acid, highly specific for 1-acyl-sn-glycerol 3-phosphate, catalyzes acylation of position 2 hydroxyl-group
487184
Pisum sativum
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
i.e. phosphatidic acid
487184
Pisum sativum
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
unsaturated preferred
487184
Pisum sativum
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
i.e. phosphatidic acid
487184
Pisum sativum
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
pronounced specificities and selectivities for unsaturated C18-CoA thioesters
487184
Pisum sativum
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
i.e. phosphatidic acid
487184
Pisum sativum
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
involved in phospholipid biosynthesis
487184
Pisum sativum
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
?
2.3.1.51
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
1-oleoyl-sn-glycerol 3-phosphate is the best acceptor
487184
Pisum sativum
CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
?
2.3.1.51
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
-
2.3.1.51
acyl-CoA + 2-acyl-sn-glycerol 3-phosphate
the enzyme does not acylate 2-acyl-sn-glycerol 3-phosphate
487184
Pisum sativum
CoA + ?
-
-
-
-
2.3.1.51
acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
CoA + ?
-
487184
Pisum sativum
-
2.3.1.51
acyl-CoA + myristoyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
CoA + ?
-
487184
Pisum sativum
-
2.3.1.51
gondoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
gondoyl-CoA is utilized less efficiently for the reaction than oleoyl-CoA
487184
Pisum sativum
CoA + 1-acyl-2-gondoyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
?
2.3.1.51
additional information
the microsomal enzyme displays a specificity for unsaturated C18-CoA thioesters and this fatty-acid specificity is not altered in the course of the purification procedure
487184
Pisum sativum
?
-
-
-
-
2.3.1.51
additional information
acyl-CoA thioesters can be considered as the physiological substrates of the microsomal enzyme
487184
Pisum sativum
?
-
-
-
-
2.3.1.51
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
oleoyl-CoA is the best substrate, both membrane-bound and partially purified enzyme
487184
Pisum sativum
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
?
2.3.1.51
palmitoyl-ACP + 1-acyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
ACP + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
?
2.3.1.51
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
487184
Pisum sativum
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.3.1.51
25
-
assay at
Pisum sativum