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Literature summary extracted from

  • Takechi, S.; Nakayama, T.
    Sas3 is a histone acetyltransferase and requires a zinc finger motif (1999), Biochem. Biophys. Res. Commun., 266, 405-410.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.48 expression of wild-type Sas3 protein and mutants as GST-fusion proteins in Escherichia coli Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.48 C303A site-directed mutagenesis of Sas3, exchange in zinc finger motif, remaining activity: 4.5% of wild-type activity Saccharomyces cerevisiae
2.3.1.48 C306A site-directed mutagenesis of Sas3, exchange in zinc finger motif, remaining activity: 11.1% of wild-type activity Saccharomyces cerevisiae
2.3.1.48 C323A site-directed mutagenesis of Sas3, exchange in zinc finger motif, no remaining activity Saccharomyces cerevisiae
2.3.1.48 G429E site-directed mutagenesis of Sas3, exchange in acetyl-CoA binding motif, no remaining activity Saccharomyces cerevisiae
2.3.1.48 G431A site-directed mutagenesis of Sas3, exchange in acetyl-CoA binding motif, nearly no remaining activity: 0.8% of wild-type activity Saccharomyces cerevisiae
2.3.1.48 H319A site-directed mutagenesis of Sas3, exchange in zinc finger motif, nearly no remaining activity: 1.2% of wild-type activity Saccharomyces cerevisiae
2.3.1.48 K428A site-directed mutagenesis of Sas3, exchange in acetyl-CoA binding motif, remaining activity: 55.9% of wild-type activity Saccharomyces cerevisiae
2.3.1.48 Q426A site-directed mutagenesis of Sas3, exchange in acetyl-CoA binding motif, remaining activity: 38.6% of wild-type activity Saccharomyces cerevisiae
2.3.1.48 Y430A site-directed mutagenesis of Sas3, exchange in acetyl-CoA binding motif, remaining activity: 16.7% of wild-type activity Saccharomyces cerevisiae
2.3.1.48 Y430L site-directed mutagenesis of Sas3, exchange in acetyl-CoA binding motif, remaining activity: 87.0% of wild-type activity Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.48 Saccharomyces cerevisiae
-
 Sas3 gene
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.48 recombinant wild-type Sas3 protein and mutants as GST-fusion proteins from E. coli Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.48 acetyl-CoA + histone
-
 Saccharomyces cerevisiae CoA + acetylhistone
-
 ?
2.3.1.48 acetyl-CoA + histone H2A
-
 Saccharomyces cerevisiae CoA + acetylhistone H2A
-
 ?
2.3.1.48 acetyl-CoA + histone H3
-
 Saccharomyces cerevisiae CoA + acetylhistone H3
-
 ?
2.3.1.48 acetyl-CoA + histone H4 histone H4 is the preferred substrate Saccharomyces cerevisiae CoA + acetylhistone H4
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.48 HAT
-
 Saccharomyces cerevisiae
2.3.1.48 More enzymes with histone acetyltransferase and transcription cofactor activity belong to the MYST protein family Saccharomyces cerevisiae