EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.47 | 1,10-phenanthroline | to some extent | Lysinibacillus sphaericus | |
2.3.1.47 | Ag+ | - |
Lysinibacillus sphaericus | |
2.3.1.47 | Cd2+ | weak | Lysinibacillus sphaericus | |
2.3.1.47 | citrate | to some extent | Lysinibacillus sphaericus | |
2.3.1.47 | Co2+ | - |
Lysinibacillus sphaericus | |
2.3.1.47 | Cu2+ | - |
Lysinibacillus sphaericus | |
2.3.1.47 | D-alanine | - |
Lysinibacillus sphaericus | |
2.3.1.47 | D-cycloserine | - |
Lysinibacillus sphaericus | |
2.3.1.47 | D-penicillamine | L-enantiomer not as effective | Lysinibacillus sphaericus | |
2.3.1.47 | D/L-histidine | - |
Lysinibacillus sphaericus | |
2.3.1.47 | diisopropyl fluorophosphate | to some extent | Lysinibacillus sphaericus | |
2.3.1.47 | glycine | - |
Lysinibacillus sphaericus | |
2.3.1.47 | hydroxylamine | - |
Lysinibacillus sphaericus | |
2.3.1.47 | isoniazide | to some extent | Lysinibacillus sphaericus | |
2.3.1.47 | L-cysteine | strong | Lysinibacillus sphaericus | |
2.3.1.47 | L-serine | - |
Lysinibacillus sphaericus | |
2.3.1.47 | additional information | no inhibition by HgCl2, EDTA, 2,2'-dipyridyl, oxalate, KCN, various amino acids | Lysinibacillus sphaericus | |
2.3.1.47 | Ni2+ | - |
Lysinibacillus sphaericus | |
2.3.1.47 | phenylhydrazine | - |
Lysinibacillus sphaericus | |
2.3.1.47 | Semicarbazide | - |
Lysinibacillus sphaericus | |
2.3.1.47 | Zn2+ | weak | Lysinibacillus sphaericus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.47 | additional information | no metal ion requirement | Lysinibacillus sphaericus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.47 | 6-carboxyhexanoyl-CoA + L-alanine | Lysinibacillus sphaericus | i.e. pimeloyl-CoA | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ? | |
2.3.1.47 | 6-carboxyhexanoyl-CoA + L-alanine | Lysinibacillus sphaericus | reaction in biotin biosynthesis | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.47 | Lysinibacillus sphaericus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.47 | partial | Lysinibacillus sphaericus |
2.3.1.47 | recombinant from E. coli overproducing strain | Lysinibacillus sphaericus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.47 | 0.00225 | - |
partially purified enzyme | Lysinibacillus sphaericus |
EC Number | Storage Stability | Organism |
---|---|---|
2.3.1.47 | -20°C, 0.01 M phosphate buffer, pH 7.0, 5 mM 2-mercaptoethanol, 5 months | Lysinibacillus sphaericus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.47 | 6-carboxyhexanoyl-CoA + L-alanine | absolutely specific for L-alanine | Lysinibacillus sphaericus | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ? | |
2.3.1.47 | 6-carboxyhexanoyl-CoA + L-alanine | i.e. pimeloyl-CoA | Lysinibacillus sphaericus | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ? | |
2.3.1.47 | 6-carboxyhexanoyl-CoA + L-alanine | reaction in biotin biosynthesis | Lysinibacillus sphaericus | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.47 | KAPA synthetase | - |
Lysinibacillus sphaericus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.47 | 37 | - |
assay at | Lysinibacillus sphaericus |
2.3.1.47 | 60 | - |
- |
Lysinibacillus sphaericus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.47 | 45 | 65 | about half-maximal activity at 45°C and 65°C | Lysinibacillus sphaericus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.47 | 60 | - |
and below, stable | Lysinibacillus sphaericus |
2.3.1.47 | 70 | - |
t1/2: 10 min | Lysinibacillus sphaericus |
2.3.1.47 | 80 | - |
and above, inactivation | Lysinibacillus sphaericus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.47 | 7 | - |
- |
Lysinibacillus sphaericus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.47 | 6.5 | 7.5 | about half-maximal activity at pH 6.5 and pH 7.5 | Lysinibacillus sphaericus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.47 | pyridoxal 5'-phosphate | requirement, pyridoxal-5'-phosphate protein, not replaceable by pyridoxal, pyridoxine, pyridoxine 5-phosphate, pyridoxamine, pyridoxamine 5-phosphate | Lysinibacillus sphaericus |