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Literature summary extracted from

  • Shimakata, T.; Stumpf, P.L.
    Purification and characterization of beta-ketoacyl-[acyl-carrier-protein] synthetase I from Spinacia oleracea leaves (1983), Arch. Biochem. Biophys., 220, 39-45.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.41 arsenite weak at 1 mM Spinacia oleracea
2.3.1.41 cerulenin antibiotic, protection by hexanoyl-[acyl-carrier-protein], decanoyl-[acyl-carrier-protein], and tetradecanoyl-[acyl-carrier-protein] Spinacia oleracea
2.3.1.41 N-ethylmaleimide 5 mM Spinacia oleracea
2.3.1.41 p-chloromercuribenzoate complete inhibition at 1 mM Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.41 additional information
-
 additional information
-
 Spinacia oleracea
2.3.1.41 0.004
-
 malonyl-CoA in presence of [acyl-carrier-protein] Spinacia oleracea
2.3.1.41 0.0075
-
 hexanoyl-[acyl-carrier protein]
-
 Spinacia oleracea
2.3.1.41 0.0091
-
 tetradecanoyl-[acyl-carrier protein]
-
 Spinacia oleracea
2.3.1.41 0.016
-
 malonyl-CoA without [acyl-carrier-protein] Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.41 56000
-
 synthase I, gel filtration Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.41 Spinacia oleracea
-
 synthase I and II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.41 synthase I Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.41 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.41 0.0083
-
 purified enzyme Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
2.3.1.41 -20°C, t1/2: 1 month Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.41 decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Spinacia oleracea dodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] poor substrate Spinacia oleracea 3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] i.e. palmitoyl-[acyl-carrier-protein] Spinacia oleracea 3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] best substrate, synthase I Spinacia oleracea 3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 malonyl-CoA + decanoyl-[acyl carrier protein]
-
 Spinacia oleracea ?
-
 ?
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] substrate specificity, overview Spinacia oleracea 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] not octodecanoyl-[acyl-carrier-protein], i.e stearoyl-[acyl-carrier-protein] Spinacia oleracea 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Spinacia oleracea 3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.41 25
-
 assay at Spinacia oleracea
2.3.1.41 37
-
 assay at Spinacia oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.41 6 8.2
-
 Spinacia oleracea