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Literature summary extracted from

  • Pattabiraman, T.N.; Bachhawat, B.K.
    Purification of glucosamine-6-phosphate N-acetylase from sheep brain (1962), Biochim. Biophys. Acta, 59, 681-689.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.4 EDTA activates Ovis aries
2.3.1.4 free sulfhydryl groups activate Ovis aries

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.4 acetyl-CoA high concentrations Ovis aries
2.3.1.4 EDTA
-
 Neurospora crassa
2.3.1.4 Ni2+
-
 Ovis aries
2.3.1.4 p-chloromercuribenzoate
-
 Neurospora crassa
2.3.1.4 p-chloromercuribenzoate 0.001 mM, 50% inhibition, 0.01 mM, complete inhibition, reversed by cysteine Ovis aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.4 0.25
-
 acetyl-CoA
-
 Ovis aries
2.3.1.4 0.45
-
 D-glucosamine 6-phosphate
-
 Ovis aries

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.4 Mg2+ slight but consistent enhancing effect Ovis aries
2.3.1.4 Mn2+ slight but consistent enhancing effect Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.4 Neurospora crassa
-
-
-
2.3.1.4 Ovis aries
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.4 ammonium sulfate, calcium phosphate gel, heat treatment at 65°C Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.4 brain
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.4 0.0054
-
-
 Ovis aries

Storage Stability

EC Number Storage Stability Organism
2.3.1.4 frozen state, 2 weeks, 50% loss of activity Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.4 acetyl-CoA + D-glucosamine 6-phosphate
-
 Neurospora crassa CoA + N-acetyl-D-glucosamine 6-phosphate
-
 ?
2.3.1.4 acetyl-CoA + D-glucosamine 6-phosphate
-
 Ovis aries CoA + N-acetyl-D-glucosamine 6-phosphate
-
 ?
2.3.1.4 propionyl-CoA + D-glucosamine 6-phosphate 15% of activity with acetyl-CoA Ovis aries CoA + N-propionyl-D-glucosamine 6-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.4 6 7.1
-
 Neurospora crassa
2.3.1.4 7.4
-
-
 Ovis aries

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.4 6 8 approx. 90% of maximal activity at pH 6.0, approx. 95% of maximal activity at pH 8.0 Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.4 additional information no cofactor required Neurospora crassa