Literature summary extracted from
Rangan, V.S.; Serre, L.; Witkowska, H.E.; Bari, A.; Smith, S.
Characterization of the malonyl-/acetyltransacylase domain of the multifunctional animal fatty acid synthase by expression in Escherichia coli and refolding in vitro (1997), Protein Eng., 10, 561-566.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.3.1.39 |
expression in Escherichia coli of cDNAs of various lengths encoding the second domain of EC 2.3.1.85. Expression of a shortened transacylase, consisting of EC 2.3.1.85 residues 488-809 in Spodoptera frugiperda |
Rattus norvegicus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.3.1.39 |
Rattus norvegicus |
- |
malonyl transferase domain of EC 2.3.1.85 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.3.1.39 |
of cytosolic recombinant transacylase, resulting from the expression of DNA corresponding to residues 488-809 of EC 2.3.1.85 in Sf9 cells, purified with column chromatography on DE-53 anion exchanger, high-performance liquid chromatography on an Ultraspherogel SEC3000 and gel filtration |
Rattus norvegicus |
Renatured (Commentary)
EC Number |
Renatured (Comment) |
Organism |
---|
2.3.1.39 |
malonyl transferase domain of EC 2.3.1.85: refolding in vitro of the recombinant protein expressed in E. coli. A shortened transacylase, consisting of EC 2.3.1.85 residues 488-409 can be repeatedly denatured and renatured in vitro with reproducible high recovery and no loss in specific activity |
Rattus norvegicus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.3.1.39 |
malonyl-CoA + pantetheine |
reaction catalysed by the malonyl transferase domain of EC 2.3.1.85 |
Rattus norvegicus |
malonyl-pantetheine + CoA |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.3.1.39 |
monomer |
malonyl transferase domain of EC 2.3.1.85, gel filtration |
Rattus norvegicus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.3.1.39 |
More |
in vertebrates, yeast and mycobacteria malonyl transferase activity is a domain of the multifunctional polypeptide chains of fatty acid synthase EC 2.3.1.85 and EC 2.3.1.86 |
Rattus norvegicus |