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Literature summary extracted from

  • Prescott, D.J.; Vagelos, P.R.
    Acyl carrier protein (1972), Adv. Enzymol. Relat. Areas Mol. Biol., 36, 269-311.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.39 dithiothreitol stimulates Escherichia coli
2.3.1.41 2-mercaptoethanol activation Escherichia coli
2.3.1.41 phosphate activation, 0.2 M Escherichia coli

Application

EC Number Application Comment Organism
2.7.8.7 analysis enzyme can be utilized in an assay for apo-ACP in biological material Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.41
-
 Escherichia coli

General Stability

EC Number General Stability Organism
2.7.8.7 very unstable, markedly protected from inactivation by the presence of half-saturating concentrations of reduced CoA Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.38 iodoacetamide acetyl-CoA protects Escherichia coli
2.3.1.38 N-ethylmaleimide acetyl-CoA protects Escherichia coli
2.3.1.39 additional information no inhibition by sulfhydryl inhibitors Escherichia coli
2.3.1.39 p-chloromercuribenzoate strong Escherichia coli
2.3.1.39 phenylmethanesulfonyl fluoride
-
 Escherichia coli
2.3.1.41 iodoacetamide
-
 Escherichia coli
2.3.1.41 N-ethylmaleimide
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.41 additional information
-
 additional information
-
 Escherichia coli
2.7.8.7 0.0004
-
 apo-acyl-carrier protein
-
 Escherichia coli
2.7.8.7 0.15
-
 CoA pH 8.0, 33°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.41 cytosol
-
 Escherichia coli 5829
-
2.7.8.7 cytoplasm
-
 Escherichia coli 5737
-
2.7.8.7 cytoplasm
-
 Saccharomyces cerevisiae 5737
-
2.7.8.7 cytoplasm
-
 Clostridium kluyveri 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.7 Mg2+ maximal activity at 0.025 mM Escherichia coli
2.7.8.7 additional information CuSO4, CdCl2 and CrCl2 does not stimulate the reaction at any ceoncentration, trivalent metal cations such as FeCl3 or AlCl3 are ineffective Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.41 34000
-
 2 * 34000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.39 malonyl-CoA + acyl-carrier protein Escherichia coli initial reaction in de novo fatty acid synthesis, part of non-associated fatty acid synthase system of plants and prokaryotes CoA + malonyl-acyl-carrier protein
-
 ?
2.7.8.7 CoA + apo-[acyl-carrier protein] Escherichia coli
-
 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
2.7.8.7 CoA + apo-[acyl-carrier protein] Saccharomyces cerevisiae
-
 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.38 Escherichia coli
-
-
-
2.3.1.39 Escherichia coli
-
-
-
2.3.1.41 Escherichia coli
-
-
-
2.7.8.7 Clostridium kluyveri
-
-
-
2.7.8.7 Escherichia coli
-
-
-
2.7.8.7 Rattus norvegicus
-
 rat
-
2.7.8.7 Saccharomyces cerevisiae
-
 yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.39 DEAE-cellulose and DEAE-Sephadex chromatography Escherichia coli
2.3.1.41
-
 Escherichia coli
2.7.8.7 partially Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.39 malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] mechanism, formation of a malonyl-enzyme intermediate Escherichia coli
a
2.3.1.41 an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] mechanism Escherichia coli
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.8.7 liver
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.3.1.41 -20°C, 90% loss of activity within 1 year Escherichia coli
2.3.1.41 -20°C, at least 1 month Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.38 acetyl-CoA + [acyl-carrier protein] acetyl-S-pantetheine can replace acetyl-CoA Escherichia coli CoA + acetyl-[acyl-carrier protein]
-
 ?
2.3.1.38 butyryl-CoA + [acyl-carrier protein]
-
 Escherichia coli CoA + butyryl-[acyl-carrier protein]
-
 ?
2.3.1.38 hexanoyl-CoA + [acyl-carrier protein]
-
 Escherichia coli CoA + hexanoyl-[acyl-carrier protein]
-
 ?
2.3.1.38 additional information in vertebrates, yeast and Mycobacteria acetyl transferase activity is a domain of the multifunctional polypeptide chains of fatty acid synthase EC 2.3.1.85 and EC 2.3.1.86 Escherichia coli ?
-
 ?
2.3.1.38 octanoyl-CoA + [acyl-carrier protein]
-
 Escherichia coli CoA + octanoyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein acetyl-CoA cannot replace malonyl-CoA Escherichia coli CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein characterization of acyl-carrier protein Escherichia coli CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein via stable covalent malonyl-enzyme intermediate Escherichia coli CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein initial reaction in de novo fatty acid synthesis, part of non-associated fatty acid synthase system of plants and prokaryotes Escherichia coli CoA + malonyl-acyl-carrier protein
-
 ?
2.3.1.39 malonyl-CoA + pantetheine
-
 Escherichia coli malonyl-pantetheine + CoA
-
 ?
2.3.1.39 malonyl-pantetheine + acyl-carrier protein
-
 Escherichia coli pantetheine + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.41 acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Escherichia coli acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 cis-mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Escherichia coli cis-mono-unsaturated 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] not hexadecanoyl-[acyl-carrier-protein] Escherichia coli 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] specific for acyl-[acyl-carrier-protein] thioesters, not acyl-CoA or acyl pantetheine thioesters Escherichia coli 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.7.8.7 CoA + apo-[acyl-carrier protein]
-
 Escherichia coli adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
2.7.8.7 CoA + apo-[acyl-carrier protein]
-
 Saccharomyces cerevisiae adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
2.7.8.7 additional information specificity of the holo-ACP synthetase is not examined in detail, only CoA is the donor of the 4'-phosphopantetheine moiety, dephospho-CoA is essentially inactive Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.38 More vertebrates, yeast and Mycobacteria fatty acid synthetases contain all individual activities on one or two multifunctional polypeptide chains, plant, Escherichia coli and other prokaryotic fatty acid synthases are non-associated systems of individual enzymes Escherichia coli
2.3.1.41 dimer 2 * 34000, SDS-PAGE Escherichia coli
2.3.1.41 More vertebrates, yeast and Mycobacteria fatty acid synthetases contain all individual activities on one or two multifunctional polypeptide chains, plant, Escherichia coli and other prokaryotic fatty acid synthases are non-associated systems of individual enzymes Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.3.1.41 More in vertebrates, yeast and Mycobacteria beta-ketoacyl synthase activity is a domain of the multifunctional polypeptide chains of fatty acid synthase EC 2.3.1.85 and EC 2.3.1.86 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.41 30
-
 assay at Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.41 100
-
 inactivation after 1 min Escherichia coli