BRENDA - Enzyme Database show

Active site of 5-aminolevulinate synthase resides at the subunit interface. Evidence from in vivo heterodimer formation

Tan, D.; Ferreira, G.C.; Biochemistry 35, 8934-8941 (1996)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.3.1.37
expression of His-tagged wild-type, mutant K313A, mutant R149A and dimer mutant K313A/R149A, each subunit from 1 plasmid, in Escherichia coli hemA- mutant
Mus musculus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.3.1.37
C145R
mutant strain G205, lacks enzyme activity, but can complement mutant strain Ole3 with mutation G344C
Saccharomyces cerevisiae
2.3.1.37
K313A
mutation site located at the active site of 1 subunit, functional complementation of Escherichia coli mutant strain hemA-, no activity
Mus musculus
2.3.1.37
K313A/R149A
each mutation site located on 1 subunit, 2 plasmids, coexpression of the dimer in Escherichia coli hemA-, functional complementation, 26% activity compared to wild-type
Mus musculus
2.3.1.37
N157Y/N162S
mutant strain G101, lacks enzyme activity, but can complement mutant strain G220 with mutation T452R
Saccharomyces cerevisiae
2.3.1.37
R149A
mutation site located at the active site of 1 subunit, functional complementation of Escherichia coli mutant strain hemA-, no activity
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
1.52
-
succinyl-CoA
recombinant mutant dimer K149A/K313A
Mus musculus
2.3.1.37
1.82
-
succinyl-CoA
-
Mus musculus
2.3.1.37
6.95
-
glycine
recombinant mutant dimer K149A/K313A
Mus musculus
2.3.1.37
8.39
-
glycine
-
Mus musculus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
additional information
-
amino acid sequence alignment
Mus musculus
2.3.1.37
additional information
-
amino acid sequence alignment
Saccharomyces cerevisiae
2.3.1.37
56000
-
2 * 56000, SDS-PAGE
Mus musculus
2.3.1.37
112000
-
gel filtration
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.37
succinyl-CoA + glycine
Mus musculus
rate-limiting enzyme of heme biosynthesis
5-aminolevulinate + CoA + CO2
-
Mus musculus
?
2.3.1.37
succinyl-CoA + glycine
Saccharomyces cerevisiae
rate-limiting enzyme of heme biosynthesis
5-aminolevulinate + CoA + CO2
-
Saccharomyces cerevisiae
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.37
Mus musculus
-
gene hemA
-
2.3.1.37
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.37
recombinant of His-tagged wild-type and mutants from E. coli
Mus musculus
Reaction
EC Number
Reaction
Commentary
Organism
2.3.1.37
succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2
the active site is located at the subunit interface and contains catalytically essential residues from the two subunits; Tyr121, Asp279, Arg439, and Lys313 are involved in substrate and cofactor binding, mechanism, subunit localisation
Mus musculus
2.3.1.37
succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2
the active site is located at the subunit interface and contains catalytically essential residues from the two subunits
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486852
Mus musculus
5-aminolevulinate + CoA + CO2
-
486852
Mus musculus
?
2.3.1.37
succinyl-CoA + glycine
-
486852
Saccharomyces cerevisiae
5-aminolevulinate + CoA + CO2
-
486852
Saccharomyces cerevisiae
?
2.3.1.37
succinyl-CoA + glycine
rate-limiting enzyme of heme biosynthesis
486852
Mus musculus
5-aminolevulinate + CoA + CO2
-
486852
Mus musculus
?
2.3.1.37
succinyl-CoA + glycine
rate-limiting enzyme of heme biosynthesis
486852
Saccharomyces cerevisiae
5-aminolevulinate + CoA + CO2
-
486852
Saccharomyces cerevisiae
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.37
dimer
-
Saccharomyces cerevisiae
2.3.1.37
dimer
2 * 56000, SDS-PAGE
Mus musculus
2.3.1.37
More
the active site is located at the subunit interface and contains catalytically essential residues from the two subunits
Mus musculus
2.3.1.37
More
the active site is located at the subunit interface and contains catalytically essential residues from the two subunits
Saccharomyces cerevisiae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
-
Saccharomyces cerevisiae
2.3.1.37
pyridoxal 5'-phosphate
-
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
expression of His-tagged wild-type, mutant K313A, mutant R149A and dimer mutant K313A/R149A, each subunit from 1 plasmid, in Escherichia coli hemA- mutant
Mus musculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
-
Saccharomyces cerevisiae
2.3.1.37
pyridoxal 5'-phosphate
-
Mus musculus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.3.1.37
C145R
mutant strain G205, lacks enzyme activity, but can complement mutant strain Ole3 with mutation G344C
Saccharomyces cerevisiae
2.3.1.37
K313A
mutation site located at the active site of 1 subunit, functional complementation of Escherichia coli mutant strain hemA-, no activity
Mus musculus
2.3.1.37
K313A/R149A
each mutation site located on 1 subunit, 2 plasmids, coexpression of the dimer in Escherichia coli hemA-, functional complementation, 26% activity compared to wild-type
Mus musculus
2.3.1.37
N157Y/N162S
mutant strain G101, lacks enzyme activity, but can complement mutant strain G220 with mutation T452R
Saccharomyces cerevisiae
2.3.1.37
R149A
mutation site located at the active site of 1 subunit, functional complementation of Escherichia coli mutant strain hemA-, no activity
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.37
1.52
-
succinyl-CoA
recombinant mutant dimer K149A/K313A
Mus musculus
2.3.1.37
1.82
-
succinyl-CoA
-
Mus musculus
2.3.1.37
6.95
-
glycine
recombinant mutant dimer K149A/K313A
Mus musculus
2.3.1.37
8.39
-
glycine
-
Mus musculus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
additional information
-
amino acid sequence alignment
Mus musculus
2.3.1.37
additional information
-
amino acid sequence alignment
Saccharomyces cerevisiae
2.3.1.37
56000
-
2 * 56000, SDS-PAGE
Mus musculus
2.3.1.37
112000
-
gel filtration
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.37
succinyl-CoA + glycine
Mus musculus
rate-limiting enzyme of heme biosynthesis
5-aminolevulinate + CoA + CO2
-
Mus musculus
?
2.3.1.37
succinyl-CoA + glycine
Saccharomyces cerevisiae
rate-limiting enzyme of heme biosynthesis
5-aminolevulinate + CoA + CO2
-
Saccharomyces cerevisiae
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
recombinant of His-tagged wild-type and mutants from E. coli
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486852
Mus musculus
5-aminolevulinate + CoA + CO2
-
486852
Mus musculus
?
2.3.1.37
succinyl-CoA + glycine
-
486852
Saccharomyces cerevisiae
5-aminolevulinate + CoA + CO2
-
486852
Saccharomyces cerevisiae
?
2.3.1.37
succinyl-CoA + glycine
rate-limiting enzyme of heme biosynthesis
486852
Mus musculus
5-aminolevulinate + CoA + CO2
-
486852
Mus musculus
?
2.3.1.37
succinyl-CoA + glycine
rate-limiting enzyme of heme biosynthesis
486852
Saccharomyces cerevisiae
5-aminolevulinate + CoA + CO2
-
486852
Saccharomyces cerevisiae
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.37
dimer
-
Saccharomyces cerevisiae
2.3.1.37
dimer
2 * 56000, SDS-PAGE
Mus musculus
2.3.1.37
More
the active site is located at the subunit interface and contains catalytically essential residues from the two subunits
Mus musculus
2.3.1.37
More
the active site is located at the subunit interface and contains catalytically essential residues from the two subunits
Saccharomyces cerevisiae