BRENDA - Enzyme Database show

5-Aminolevulinic-acid synthetase of Rhodopseudomonas spheroides Y. Purification and some properties

Fanica-Gaignier, M.; Clement-Metral, J.; Eur. J. Biochem. 40, 13-18 (1973)

Data extracted from this reference:

Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
105000
-
enzyme I, gel filtration
Rhodobacter sphaeroides
2.3.1.37
110000
-
enzyme II, gel filtration
Rhodobacter sphaeroides
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.37
Rhodobacter sphaeroides
-
2 isozymes; Y
-
2.3.1.37
Rhodobacter sphaeroides Y
-
Y
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.37
isoforms I and II
Rhodobacter sphaeroides
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.37
0.167
-
isozyme II
Rhodobacter sphaeroides
2.3.1.37
0.375
-
purified isozyme I
Rhodobacter sphaeroides
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.37
-15°C, 0.1 mM pyridoxal 5'-phosphate, 10% glycerol, 20% loss of activity after 6 weeks
Rhodobacter sphaeroides
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486844
Rhodobacter sphaeroides
5-aminolevulinate + CoA + CO2
-
486844
Rhodobacter sphaeroides
?
2.3.1.37
succinyl-CoA + glycine
-
486844
Rhodobacter sphaeroides Y
5-aminolevulinate + CoA + CO2
-
486844
Rhodobacter sphaeroides Y
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
additional information
-
pI
Rhodobacter sphaeroides
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
contains 1 molar equivalent of pyridoxal 5'-phosphate
Rhodobacter sphaeroides
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
contains 1 molar equivalent of pyridoxal 5'-phosphate
Rhodobacter sphaeroides
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
105000
-
enzyme I, gel filtration
Rhodobacter sphaeroides
2.3.1.37
110000
-
enzyme II, gel filtration
Rhodobacter sphaeroides
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
isoforms I and II
Rhodobacter sphaeroides
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.37
0.167
-
isozyme II
Rhodobacter sphaeroides
2.3.1.37
0.375
-
purified isozyme I
Rhodobacter sphaeroides
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.37
-15°C, 0.1 mM pyridoxal 5'-phosphate, 10% glycerol, 20% loss of activity after 6 weeks
Rhodobacter sphaeroides
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
-
486844
Rhodobacter sphaeroides
5-aminolevulinate + CoA + CO2
-
486844
Rhodobacter sphaeroides
?
2.3.1.37
succinyl-CoA + glycine
-
486844
Rhodobacter sphaeroides Y
5-aminolevulinate + CoA + CO2
-
486844
Rhodobacter sphaeroides Y
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
additional information
-
pI
Rhodobacter sphaeroides