BRENDA - Enzyme Database show

Regulation of prophyrin biosynthesis. Purification and characterization of delta-aminolevulinic acid synthase

Warnick, G.R.; Burnham, B.F.; J. Biol. Chem. 246, 6880-6885 (1971)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.37
dithiothreitol
activates
Rhodobacter sphaeroides
General Stability
EC Number
General Stability
Organism
2.3.1.37
stable to freezing and thawing
Rhodobacter sphaeroides
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
2-mercaptoethanol
slightly at high concentration, 10 mM
Rhodobacter sphaeroides
2.3.1.37
dithiothreitol
-
Rhodobacter sphaeroides
2.3.1.37
hemin
-
Rhodobacter sphaeroides
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.37
potassium ferricyanide
activates at 0.5 mM
Rhodobacter sphaeroides
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
57000
-
gel filtration
Rhodobacter sphaeroides
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.37
Rhodobacter sphaeroides
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.37
-
Rhodobacter sphaeroides
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.37
2.17
-
purified enzyme
Rhodobacter sphaeroides
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.37
-15°C, 50 mM phosphate buffer, pH 6.8, 10% glycerol, 1 mM 2-mercaptoethanol, stable for several months
Rhodobacter sphaeroides
2.3.1.37
4°C, 50 mM phosphate buffer, pH 6.8, 10% glycerol, 1 mM 2-mercaptoethanol, 10% loss of activity after 2 weeks
Rhodobacter sphaeroides
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
absolute requirement for glycine
486811
Rhodobacter sphaeroides
5-aminolevulinate + CoA + CO2
-
486811
Rhodobacter sphaeroides
?
2.3.1.37
succinyl-CoA + glycine
absolute requirement for succinyl-CoA
486811
Rhodobacter sphaeroides
5-aminolevulinate + CoA + CO2
-
486811
Rhodobacter sphaeroides
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.37
?
x * 57000, SDS-PAGE
Rhodobacter sphaeroides
2.3.1.37
monomer
1 * 57000, SDS-PAGE
Rhodobacter sphaeroides
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.3.1.37
37
-
60 min, 20% loss of activity with addition of 2-mercaptoethanol and glycerol, 60% loss of activity without addition of 2-mercaptoethanol and glycerol
Rhodobacter sphaeroides
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
7.4
7.6
-
Rhodobacter sphaeroides
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
absolute requirement
Rhodobacter sphaeroides
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.37
dithiothreitol
activates
Rhodobacter sphaeroides
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.37
pyridoxal 5'-phosphate
absolute requirement
Rhodobacter sphaeroides
General Stability (protein specific)
EC Number
General Stability
Organism
2.3.1.37
stable to freezing and thawing
Rhodobacter sphaeroides
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.37
2-mercaptoethanol
slightly at high concentration, 10 mM
Rhodobacter sphaeroides
2.3.1.37
dithiothreitol
-
Rhodobacter sphaeroides
2.3.1.37
hemin
-
Rhodobacter sphaeroides
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.37
potassium ferricyanide
activates at 0.5 mM
Rhodobacter sphaeroides
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.37
57000
-
gel filtration
Rhodobacter sphaeroides
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.37
-
Rhodobacter sphaeroides
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.37
2.17
-
purified enzyme
Rhodobacter sphaeroides
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.37
-15°C, 50 mM phosphate buffer, pH 6.8, 10% glycerol, 1 mM 2-mercaptoethanol, stable for several months
Rhodobacter sphaeroides
2.3.1.37
4°C, 50 mM phosphate buffer, pH 6.8, 10% glycerol, 1 mM 2-mercaptoethanol, 10% loss of activity after 2 weeks
Rhodobacter sphaeroides
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.37
succinyl-CoA + glycine
absolute requirement for glycine
486811
Rhodobacter sphaeroides
5-aminolevulinate + CoA + CO2
-
486811
Rhodobacter sphaeroides
?
2.3.1.37
succinyl-CoA + glycine
absolute requirement for succinyl-CoA
486811
Rhodobacter sphaeroides
5-aminolevulinate + CoA + CO2
-
486811
Rhodobacter sphaeroides
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.37
?
x * 57000, SDS-PAGE
Rhodobacter sphaeroides
2.3.1.37
monomer
1 * 57000, SDS-PAGE
Rhodobacter sphaeroides
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.3.1.37
37
-
60 min, 20% loss of activity with addition of 2-mercaptoethanol and glycerol, 60% loss of activity without addition of 2-mercaptoethanol and glycerol
Rhodobacter sphaeroides
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.37
7.4
7.6
-
Rhodobacter sphaeroides