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Literature summary extracted from

  • Warnick, G.R.; Burnham, B.F.
    Regulation of prophyrin biosynthesis. Purification and characterization of delta-aminolevulinic acid synthase (1971), J. Biol. Chem., 246, 6880-6885.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.37 dithiothreitol activates Cereibacter sphaeroides

General Stability

EC Number General Stability Organism
2.3.1.37 stable to freezing and thawing Cereibacter sphaeroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.37 2-mercaptoethanol slightly at high concentration, 10 mM Cereibacter sphaeroides
2.3.1.37 dithiothreitol
-
 Cereibacter sphaeroides
2.3.1.37 hemin
-
 Cereibacter sphaeroides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.37 potassium ferricyanide activates at 0.5 mM Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.37 57000
-
 gel filtration Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.37 Cereibacter sphaeroides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.37
-
 Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.37 2.17
-
 purified enzyme Cereibacter sphaeroides

Storage Stability

EC Number Storage Stability Organism
2.3.1.37 -15°C, 50 mM phosphate buffer, pH 6.8, 10% glycerol, 1 mM 2-mercaptoethanol, stable for several months Cereibacter sphaeroides
2.3.1.37 4°C, 50 mM phosphate buffer, pH 6.8, 10% glycerol, 1 mM 2-mercaptoethanol, 10% loss of activity after 2 weeks Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.37 succinyl-CoA + glycine absolute requirement for glycine Cereibacter sphaeroides 5-aminolevulinate + CoA + CO2
-
 ?
2.3.1.37 succinyl-CoA + glycine absolute requirement for succinyl-CoA Cereibacter sphaeroides 5-aminolevulinate + CoA + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.37 ? x * 57000, SDS-PAGE Cereibacter sphaeroides
2.3.1.37 monomer 1 * 57000, SDS-PAGE Cereibacter sphaeroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.37 37
-
 60 min, 20% loss of activity with addition of 2-mercaptoethanol and glycerol, 60% loss of activity without addition of 2-mercaptoethanol and glycerol Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.37 7.4 7.6
-
 Cereibacter sphaeroides

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.37 pyridoxal 5'-phosphate absolute requirement Cereibacter sphaeroides